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Human IL-2 Protein 4453

$120.00$400.00

Summary

  • Expression: E.coli
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ala21-Thr153
SKU: 4453parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P60568

express system

E.coli

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15, 5 – 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.

molecular weight

The protein has a predicted 15.5 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.01 EU per μg by the LAL method.

Human IL-2 Protein 4453

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Protein names
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF) (Aldesleukin)
Gene names
IL2,IL2
Protein family
IL-2 family
Mass
9606Da
Function
FUNCTION: Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:6438535). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG) (PubMed:16293754, PubMed:16477002). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3 (PubMed:7973659). In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:8580378). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways (PubMed:25142963). Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well (PubMed:6608729). Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production (PubMed:6438535). Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells. {ECO:0000269|PubMed:16293754, ECO:0000269|PubMed:16477002, ECO:0000269|PubMed:25142963, ECO:0000269|PubMed:6438535, ECO:0000269|PubMed:6608729, ECO:0000269|PubMed:7973659, ECO:0000269|PubMed:8580378}.
Subellular location
SUBCELLULAR LOCATION: Secreted.
Target Relevance information above includes information from UniProt accession : P60568
The UniProt Consortium

Data

SPR with Human IL-2 Protein
Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).
SPR with Human IL-2 Protein
Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.25µM as determined in SPR assay (Biacore T200).
SPR with Human IL-2 Protein
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Cell based assay with Human IL-2 Protein
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/mL (QC Test).
HPLC of Human IL-2 Protein
The purity of Human IL-2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human IL-2 Protein
Human IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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