Human IL-18BP Protein 4117

$120.00 – $400.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Thr31-Gly194

SKU: 4117parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O95998
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer, components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
molecular weight	The protein has a predicted MW of 18.7 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IL-18BP Protein 4117

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Cytokines were the first modern immunotherapies to produce durable responses in patients with advanced cancer,components of the interleukin-18 (IL-18) pathway are upregulated on tumour-infiltrating lymphocytes, suggesting that IL-18 therapy could enhance anti-tumour immunity. IL-18BP, a high-affinity IL-18 decoy receptor, is frequently upregulated in diverse human and mouse tumours and limits the anti-tumour activity of IL-18 in mice.
Protein names Interleukin-18-binding protein (IL-18BP) (Tadekinig-alfa)
Gene names IL18BP,IL18BP
Mass 9606Da
Function Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.
Catalytic activity #N/A
Subellular location Secreted .
Tissues Strongly expressed in heart, lung, placenta and spleen.
Post-translational modification N- and O-glycosylated. O-glycosylated with core 1-like and core 2-like glycans. O-glycan heterogeneity at Ser-53: HexHexNAc (major) and Hex2HexNAc2 (minor). N-glycan heterogeneity at Asn-103: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); N-glycan at Asn-147: dHex1Hex5HexNAc4.
Target Relevance information above includes information from UniProt accession: O95998
The UniProt Consortium

Data

Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.41 nM as determined in SPR assay (Biacore T200).

Cell based assay with Human IL-18BP Protein

Measured by its ability to inhibit the IL-18-induced response of Human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.04 - 0.08 µg/ml in the presence of 30 ng/mL of recombinant Human IL-18 (QC Test).

Human IL-18BP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.