Human IFN alpha 1 Protein 3317

$315.00 – $1,050.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Cys24-Glu189

SKU: 3317parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P01562
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
molecular weight	The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human IFN alpha 1 Protein 3317

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Protein names Interferon alpha-1/13 (IFN-alpha-1/13) (Interferon alpha-D) (LeIF D)
Gene names IFNA1; IFNA13,IFNA1; IFNA13
Protein family Alpha/beta interferon family
Mass 9606Da
Function Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Subellular location Secreted.
Target Relevance information above includes information from UniProt accession: P01562
The UniProt Consortium

Data

Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 µM as determined in SPR assay (Biacore T200) (QC Test).

Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test).

The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human IFN alpha 1 Protein

Human IFN alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.