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Human HVEM/TNFRSF14 Protein 4468

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Leu39-Val202
SKU: 4468parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q92956

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE

background

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.

molecular weight

The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human HVEM/TNFRSF14 Protein 4468

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.  Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
Protein names
Tumor necrosis factor receptor superfamily member 14 (Herpes virus entry mediator A) (Herpesvirus entry mediator A) (HveA) (Tumor necrosis factor receptor-like 2) (TR2) (CD antigen CD270)
Gene names
TNFRSF14,TNFRSF14 HVEA HVEM UNQ329/PRO509
Protein family
Tumor necrosis factor receptor superfamily
Mass
9606Da
Function
FUNCTION: Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed:10754304, PubMed:18193050, PubMed:23761635, PubMed:9462508). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044, PubMed:9153189, PubMed:9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed:10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and pro-inflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, down-regulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (By similarity) (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (By similarity) (PubMed:19915044). {ECO:0000250|UniProtKB:Q80WM9, ECO:0000269|PubMed:10754304, ECO:0000269|PubMed:18193050, ECO:0000269|PubMed:19915044, ECO:0000269|PubMed:23761635, ECO:0000269|PubMed:9153189, ECO:0000269|PubMed:9162022, ECO:0000269|PubMed:9462508}.; FUNCTION: (Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1. {ECO:0000269|PubMed:11511370, ECO:0000269|PubMed:9696799}.; FUNCTION: (Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2. {ECO:0000269|PubMed:11511370, ECO:0000269|PubMed:9696799}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19915044}; Single-pass type I membrane protein {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Widely expressed, with the highest expression in lung, spleen and thymus. Expressed in a subpopulation of B cells and monocytes (PubMed:18193050). Expressed in naive T cells (PubMed:19915044). {ECO:0000269|PubMed:18193050, ECO:0000269|PubMed:19915044}.
Structure
SUBUNIT: Interacts with TRAF2, TRAF3 and TRAF5 (PubMed:9153189, PubMed:9162022). Interacts (via CRD1/TNFR-Cys 1) with CD160; this interaction is direct (PubMed:18193050, PubMed:23761635). Interacts with LTA and TNFSF14 (PubMed:9462508). Interacts (via CRD1/TNFR-Cys 1) in cis and trans with BTLA; the cis interactions inhibits the trans interactions (PubMed:16169851, PubMed:19915044). {ECO:0000269|PubMed:16169851, ECO:0000269|PubMed:18193050, ECO:0000269|PubMed:19915044, ECO:0000269|PubMed:23761635, ECO:0000269|PubMed:9153189, ECO:0000269|PubMed:9162022, ECO:0000269|PubMed:9462508}.; SUBUNIT: (Microbial infection) Interacts with herpes simplex virus 1/HHV-1 envelope glycoprotein D. {ECO:0000269|PubMed:11511370, ECO:0000269|PubMed:9696799}.; SUBUNIT: (Microbial infection) Interacts with herpes simplex virus 2/HHV-2 envelope glycoprotein D. {ECO:0000269|PubMed:11511370, ECO:0000269|PubMed:9696799}.
Post-translational modification
PTM: N-glycosylated. {ECO:0000269|PubMed:16169851}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : Q92956
The UniProt Consortium

Data

SPR with Human HVEM/TNFRSF14 Protein
Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 µM as determined in SPR assay (Biacore T200).
ELISA with Human HVEM/TNFRSF14 Protein
Immobilized Human BTLA, His Tag at 1µg/ml (100µl/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA (QC Test).
ELISA with Human HVEM/TNFRSF14 Protein
Immobilized Human LIGHT, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
SDS-PAGE gel of Human HVEM/TNFRSF14 Protein
Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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