Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein 3472

$310.00 – $1,125.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*11:01),Ile21-Met119(B2M)and VVVGADGVGK peptide

available size

Clear

SKU: 3472parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AAV53343(HLA-A*11:01)&P61769(B2M)&VVVGADGVGK
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
molecular weight	The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein 3472

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

SPR with Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein

Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag captured on CM5 Chip via Anti-His Antibody can bind HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR with an affinity constant of 14.91 nM as determined in SPR assay (Biacore T200).

ELISA with Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein

Immobilized HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) TCR at 5µg/ml(100µl/Well) on the plate. Dose response curve for Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer, His Tag with the EC50 of 0.39µg/ml determined by ELISA (QC Test).

HPLC of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Protein

The purity of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer Pr

Human HLA-A*11:01&B2M&KRAS G12D (VVVGADGVGK) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.