Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein 3873

$750.00 – $3,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide

available size

Clear

SKU: 3873parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
molecular weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein 3873

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

SPR with Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).

ELISA with Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein

Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).

HPLC of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein

The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protei

Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.