Human HGF Protein 3816

$255.00 – $850.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln32-Ser728

SKU: 3816parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P14210
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
molecular weight	The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain.The protein has a predicted MW of 79.6 kDa (after cleavage of the signal peptide). Due to glycosylation, the protein migrates to 60-65 kDa and 30-40 kDa corresponding to α chain and β chain based on Bis-Tris PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.1EU per μg by the LAL method.

Human HGF Protein 3816

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Hepatocyte growth factor (HGF) is an important component of the pathophysiology of IR, with increased levels in most common IR conditions, including obesity. HGF has a role in the metabolic flux of glucose in different insulin sensitive cell types.
Protein names Hepatocyte growth factor (Hepatopoietin-A) (Scatter factor) (SF) [Cleaved into: Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain]
Gene names HGF,HGF HPTA
Protein family Peptidase S1 family, Plasminogen subfamily
Mass 9606Da
Function Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types (PubMed:20624990). Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization (PubMed:15167892, PubMed:20977675). Activates MAPK signaling following TMPRSS13 cleavage and activation (PubMed:20977675).
Catalytic activity #N/A
Structure Dimer of an alpha chain and a beta chain linked by a disulfide bond. Interacts with SRPX2; the interaction increases HGF mitogenic activity.
Post-translational modification The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form.
Target Relevance information above includes information from UniProt accession: P14210
The UniProt Consortium

Data

Immobilized Human HGF, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human HGF R, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

The purity of Human HGF Protein is greater than 95% as determined by SEC-HPLC.

Human HGF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.