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Human Her3/ErbB3 Protein 5192

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Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ser20-Thr643
SKU: 5192parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P21860

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

molecular weight

The protein has a predicted MW of 95.4 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Her3/ErbB3 Protein 5192

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).
Protein names
Receptor tyrosine-protein kinase erbB-3 (EC 2.7.10.1) (Proto-oncogene-like protein c-ErbB-3) (Tyrosine kinase-type cell surface receptor HER3)
Gene names
ERBB3,ERBB3 HER3
Protein family
Protein kinase superfamily, Tyr protein kinase family, EGF receptor subfamily
Mass
9606Da
Function
FUNCTION: Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908). {ECO:0000269|PubMed:15358134, ECO:0000269|PubMed:20682778, ECO:0000269|PubMed:27416908}.
Catalytic activity
BINDING 715..723; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; BINDING 742; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; BINDING 788..790; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; BINDING 834..839; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"
Subellular location
SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane {ECO:0000269|PubMed:33497358}; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform 2]: Secreted.
Tissues
TISSUE SPECIFICITY: Epithelial tissues and brain.
Structure
SUBUNIT: Monomer and homodimer. Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 (PubMed:15358134). Interacts with GRB7 (PubMed:9516479). Interacts with MUC1 (PubMed:12939402). Interacts with MYOC (By similarity). Interacts with isoform 2 of PA2G4 (PubMed:11325528, PubMed:16832058). Found in a ternary complex with NRG1 and ITGAV:ITGB3 or ITGA6:ITGB4 (PubMed:20682778). {ECO:0000250|UniProtKB:Q61526, ECO:0000269|PubMed:11325528, ECO:0000269|PubMed:12939402, ECO:0000269|PubMed:15358134, ECO:0000269|PubMed:16832058, ECO:0000269|PubMed:20682778, ECO:0000269|PubMed:9516479, ECO:0000305}.
Post-translational modification
PTM: Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). {ECO:0000269|PubMed:20351256, ECO:0000269|PubMed:20682778, ECO:0000269|PubMed:33497358}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : P21860
The UniProt Consortium

Data

ELISA with Human Her3/ErbB3 Protein
Immobilized Human Her3, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-Her3 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA (QC Test).
ELISA with Human Her3/ErbB3 Protein
Immobilized Human Her3, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human NRG1 Beta 1, hFc Tag with the EC50 of 0.25µg/ml determined by ELISA.
HPLC of Human Her3/ErbB3 Protein
The purity of Human Her3 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Her3/ErbB3 Protein
Human Her3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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