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Human GM-CSF Protein 4447

$225.00$750.00

Summary

  • Expression: E.coli
  • Active: Yes (cell based assay)
  • Amino Acid Range: Ala18-Glu144
SKU: 4447parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P04141

express system

E.coli

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.

molecular weight

The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.1 EU per μg by the LAL method.

Human GM-CSF Protein 4447

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Protein names
Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF) (Molgramostin) (Sargramostim)
Gene names
CSF2,CSF2 GMCSF
Protein family
GM-CSF family
Mass
9606Da
Function
FUNCTION: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. {ECO:0000269|PubMed:3925454}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1737041, ECO:0000269|PubMed:3925454}.
Structure
SUBUNIT: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. {ECO:0000269|PubMed:18692472}.
Target Relevance information above includes information from UniProt accession : P04141
The UniProt Consortium

Data

SPR with Human GM-CSF Protein
Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).
ELISA with Human GM-CSF Protein
Immobilized Human GM-CSF at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).
Cell based assay with Human GM-CSF Protein
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).
HPLC of Human GM-CSF Protein
The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human GM-CSF Protein
Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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