Cell based assay with Human GM-CSF Protein 4447

Human GM-CSF Protein 4447

$225.00 – $750.00

Summary

Expression: E.coli
Active: Yes (cell based assay)
Amino Acid Range: Ala18-Glu144

SKU: 4447parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P04141
express system	E.coli
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
molecular weight	The protein has a predicted MW of 14.6 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.1 EU per μg by the LAL method.

Human GM-CSF Protein 4447

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Granulocyte-macrophage colony-stimulating factor (GM-CSF), also known as colony-stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, natural killer cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim.
Protein names Granulocyte-macrophage colony-stimulating factor (GM-CSF) (Colony-stimulating factor) (CSF) (Molgramostin) (Sargramostim)
Gene names CSF2,CSF2 GMCSF
Protein family GM-CSF family
Mass 9606Da
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Subellular location Secreted .
Structure Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits.
Target Relevance information above includes information from UniProt accession: P04141
The UniProt Consortium

Data

Human GM-CSF R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human GM-CSF, No Tag with an affinity constant of 0.79nM as determined in SPR assay (Biacore T200).

Immobilized Human GM-CSF at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human GM-CSF R alpha, hFc Tag with the EC50 of 11.0ng/ml determined by ELISA (QC Test).

Cell based assay with Human GM-CSF Protein

The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.02-0.1 ng/mL (QC Test).

The purity of Human GM-CSF is greater than 95% as determined by SEC-HPLC.

Human GM-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.