Human GFRA3 Protein 3633

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp32-Trp382

SKU: 3633parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O60609
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
molecular weight	The protein has a predicted MW of 40.3 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human GFRA3 Protein 3633

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GDNF (glial-cell-line derived neurotrophic factor) is a potent neurotrophic factor for dopaminergic neurons. Neuropsychiatric diseases and their treatments are associated with alterations in the levels of both GDNF and its receptor family (GDNF family receptor alpha or GFRA). GFRA1, GFRA2 and GFRA3 are located in chromosomal regions with suggestive linkage to schizophrenia.
Protein names GDNF family receptor alpha-3 (GDNF receptor alpha-3) (GDNFR-alpha-3) (GFR-alpha-3)
Gene names GFRA3,GFRA3 UNQ339/PRO538/PRO3664
Protein family GDNFR family
Mass 9606Da
Function Receptor for artemin (ARTN), a growth factor that supports the survival of sensory and sympathetic peripheral neurons (PubMed:31535977, PubMed:9883723). ARTN-binding leads to autophosphorylation and activation of the RET receptor (PubMed:31535977, PubMed:9883723).
Catalytic activity #N/A
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor .
Tissues Widely expressed in adult and fetus which exhibit a similar pattern. Essentially not expressed in the central nervous system, but highly expressed in several sensory and sympathetic ganglia of the peripheral nervous system. Moderate expression in many non-neuronal tissues, particularly those of the digestive and urogenital systems, but high expression in stomach and appendix. Several types of glandular tissues show low expression. Very low or no expression detected in the hematopoietic system.
Structure Interacts with ARTN ligand and RET: forms a 2:2:2 ternary complex composed of ARTN ligand, GFRA3 and RET receptor (PubMed:16765900, PubMed:31535977). Interacts with SORL1 (PubMed:23333276).
Post-translational modification N-glycosylated.
Target Relevance information above includes information from UniProt accession: O60609
The UniProt Consortium

Data

Immobilized Human GFRA3, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-GFRA3 Antibody, hFc Tag with the EC50 of 7.3ng/ml determined by ELISA (QC Test).

Immobilized Human GFRA3, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human ARTN, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.

The purity of Human GFRA3 is greater than 95% as determined by SEC-HPLC.

Human GFRA3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.