Human GAS6 Protein 3207

$375.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala49-Ala678

SKU: 3207parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q14393
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
background	Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).
molecular weight	The protein has a predicted MW of 70.60 kDa. Due to glycosylation, the protein migrates to 71-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1 EU per μg by the LAL method.

Human GAS6 Protein 3207

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Growth arrest-specific 6, also known as Gas6, is a human gene encoding the Gas6 protein, which was originally found to be upregulated in growth-arrested fibroblasts. Gas6 is a member of the vitamin K-dependent family of proteins expressed in many human tissues and regulates several biological processes in cells, including proliferation, survival and migration, by binding to its receptors Tyro3, Axl and Mer (TAM).
Protein names
Growth arrest-specific protein 6 (GAS-6) (AXL receptor tyrosine kinase ligand)
Gene names
GAS6,GAS6 AXLLG
Mass
9606Da
Function
Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.; (Microbial infection) Can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry (PubMed:21501828). Plays a role in Dengue cell entry by apoptotic mimicry (PubMed:23084921). Plays a role in Vaccinia virus cell entry by apoptotic mimicry (PubMed:21501828). Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry (PubMed:17005688).
Catalytic activity
#N/A
Subellular location
Secreted .
Tissues
Plasma. Isoform 1 and isoform 2 are widely expressed, isoform 1 being expressed at higher levels than isoform 2 in most tissues. Isoform 2 is the predominant form in spleen.
Structure
Heterodimer and heterotetramer with AXL.
Post-translational modification
[Isoform 2]: Proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.; Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
Target Relevance information above includes information from UniProt accession: Q14393
The UniProt Consortium

Data

Human GAS6, His Tag immobilized on CM5 Chip can bind Human TYRO3, His Tag with an affinity constant of 2.36 µM as determined in SPR assay (Biacore T200).

Immobilized Human GAS6, His Tag at 5 µg/ml (100 µl/Well) on the plate. Dose response curve for Anti-GAS6 Antibody, hFc Tag with the EC50 of 12.3 ng/ml determined by ELISA (QC Test).

Immobilized Human GAS6, His Tag at 5 µg/ml (100 µl/Well) on the plate. Dose response curve for Human Axl, hFc Tag with the EC50 of 2.6 ng/ml determined by ELISA.

The purity of Human GAS6 is greater than 95% as determined by SEC-HPLC.

Human GAS6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.