Human FSTL3 Protein 4001

$270.00 – $900.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Met27-Val263

SKU: 4001parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O95633
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE
background	Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
molecular weight	The protein has a predicted MW of 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human FSTL3 Protein 4001

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
Protein names Follistatin-related protein 3 (Follistatin-like protein 3) (Follistatin-related gene protein)
Gene names FSTL3,FSTL3 FLRG UNQ674/PRO1308
Mass 9606Da
Function Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such as activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiation. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10.
Catalytic activity #N/A
Subellular location [Isoform 1]: Secreted.; [Isoform 2]: Nucleus. Note=Although alternative initiation has been demonstrated and resulted in different localization, the major source of nuclear FSTL3 appears not to depend on translation initiation at Met-27 according to.
Tissues Expressed in a wide range of tissues.
Structure Interacts with INHBA and INHBB. Interacts with FN1. Interacts with ADAM12. Isoform 2 interacts with MLLT10; the interaction enhances MLLT10 in vitro transcriptional activity and self-association. Interacts with MSTN.
Target Relevance information above includes information from UniProt accession: O95633
The UniProt Consortium

Data

Immobilized Human FSTL3, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA (QC Test).

Immobilized Human Activin A, No Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human FSTL3, His Tag with the EC50 of 7.6ng/ml determined by ELISA.

Cell based assay with Human FSTL3 Protein

Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 5-25 ng/mL in the presence of 7.5 ng/mL rhActivin A.

Human FSTL3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.