Human FOLR4/Juno Protein 3436

$330.00 – $1,100.00

Summary

Expression: HEK293
Pure: Yes (HPLC)
Amino Acid Range: Gly20-Ser228

SKU: 3436parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A6ND01
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
molecular weight	The protein has a predicted MW of 25 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Human FOLR4/Juno Protein 3436

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Izumo1 is the only essential sperm-egg fusion protein currently known on mammalian sperm, and its egg receptor (Juno; formerly Folr4) was recently discovered. Male knockout mice for Izumo1 and female knockout mice for Juno are both healthy but sterile. Here, both sperm-egg binding proteins are shown to be evolving under positive selection. Juno's presence in mammals alone, suggesting a recent mammalian-specific duplication and neofunctionalization of the ancestral folate receptor.
Protein names Sperm-egg fusion protein Juno (Folate receptor 4) (Folate receptor delta) (FR-delta) (IZUMO1 receptor protein JUNO)
Gene names IZUMO1R,IZUMO1R FOLR4 JUNO
Protein family Folate receptor family
Mass 9606Da
Function Receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.
Catalytic activity #N/A
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Cell projection, microvillus membrane ; Lipid-anchor, GPI-anchor. Note=GPI-anchored at the oolemma microvilli.
Tissues Expressed in unfertilized oocytes (at protein level).
Structure Monomer (PubMed:27309808). Interacts with IZUMO1; the interaction is direct. IZUMO1 and IZUMO1R/JUNO form a complex with 1:1 stoichiometry (PubMed:24739963, PubMed:27309808, PubMed:27309818). Interacts with FCRL3/MAIA; FCRL3/MAIA replaces IZUMO1R/JUNO as IZUMO1 receptor after sperm-egg adhesion, thereby permitting species-specific gamete fusion (PubMed:36070373).
Post-translational modification The protein is rapidly cleaved following fertilization, being only weakly detectable in zona-intact fertilized eggs at telophase II and undetectable at the pronuclear stage. Sheding is probably required to block to polyspermy and ensuring egg fusion with a single sperm.
Target Relevance information above includes information from UniProt accession: A6ND01
The UniProt Consortium

Data

The purity of Human FOLR4 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human FOLR4/Juno Protein

Human FOLR4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.