Human FOLR1 Protein 4499

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg25-Met233

SKU: 4499parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P15328
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
background	Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
molecular weight	The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1 EU per μg by the LAL method.

Human FOLR1 Protein 4499

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
Protein names Folate receptor alpha (FR-alpha) (Adult folate-binding protein) (FBP) (Folate receptor 1) (Folate receptor, adult) (KB cells FBP) (Ovarian tumor-associated antigen MOv18)
Gene names FOLR1,FOLR1 FOLR
Protein family Folate receptor family
Mass 9606Da
Function Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity).
Catalytic activity BINDING 103; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269\|PubMed:23851396"; BINDING 107; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269\|PubMed:23851396"; BINDING 124..128; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269\|PubMed:23851396"; BINDING 157..162; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269\|PubMed:23851396"; BINDING 196; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269\|PubMed:23851396"
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Apical cell membrane ; Lipid-anchor, GPI-anchor. Basolateral cell membrane ; Lipid-anchor, GPI-like-anchor. Secreted. Cytoplasmic vesicle. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Note=Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane.
Tissues Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium.
Post-translational modification The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease.
Target Relevance information above includes information from UniProt accession: P15328
The UniProt Consortium

Data

Immobilized Human FOLR1, His Tag at 1 µg/ml (100 µl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA.

The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC.

Human FOLR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.