no metadatahttps://benchmarkantibodies.com/product/human-folr1-protein-4499/
Skip to content

Human FOLR1 Protein 4499

$270.00$900.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Arg25-Met233
SKU: 4499parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P15328

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC

background

Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 – 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.

molecular weight

The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1 EU per μg by the LAL method.

Human FOLR1 Protein 4499

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH.
Protein names
Folate receptor alpha (FR-alpha) (Adult folate-binding protein) (FBP) (Folate receptor 1) (Folate receptor, adult) (KB cells FBP) (Ovarian tumor-associated antigen MOv18)
Gene names
FOLR1,FOLR1 FOLR
Protein family
Folate receptor family
Mass
9606Da
Function
FUNCTION: Binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells (PubMed:19074442, PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Has high affinity for folate and folic acid analogs at neutral pH (PubMed:23851396, PubMed:23934049, PubMed:2527252, PubMed:8033114, PubMed:8567728). Exposure to slightly acidic pH after receptor endocytosis triggers a conformation change that strongly reduces its affinity for folates and mediates their release (PubMed:8567728). Required for normal embryonic development and normal cell proliferation (By similarity). {ECO:0000250|UniProtKB:P35846, ECO:0000269|PubMed:19074442, ECO:0000269|PubMed:23851396, ECO:0000269|PubMed:23934049, ECO:0000269|PubMed:2527252, ECO:0000269|PubMed:8033114, ECO:0000269|PubMed:8567728}.
Catalytic activity
BINDING 103; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269|PubMed:23851396"; BINDING 107; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269|PubMed:23851396"; BINDING 124..128; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269|PubMed:23851396"; BINDING 157..162; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269|PubMed:23851396"; BINDING 196; /ligand="folate"; /ligand_id="ChEBI:CHEBI:62501"; /evidence="ECO:0000269|PubMed:23851396"
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:2527252, ECO:0000269|PubMed:8033114, ECO:0000269|PubMed:8567728}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:17566972, ECO:0000269|PubMed:7578066}. Apical cell membrane {ECO:0000269|PubMed:10787414}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:17566972, ECO:0000269|PubMed:7578066}. Basolateral cell membrane {ECO:0000269|PubMed:10787414}; Lipid-anchor, GPI-like-anchor {ECO:0000269|PubMed:17566972, ECO:0000269|PubMed:7578066}. Secreted {ECO:0000305|PubMed:3476960}. Cytoplasmic vesicle {ECO:0000269|PubMed:8567728}. Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000269|PubMed:8567728}. Endosome {ECO:0000269|PubMed:8567728}. Note=Endocytosed into cytoplasmic vesicles and then recycled to the cell membrane. {ECO:0000269|PubMed:8567728}.
Tissues
TISSUE SPECIFICITY: Primarily expressed in tissues of epithelial origin. Expression is increased in malignant tissues. Expressed in kidney, lung and cerebellum. Detected in placenta and thymus epithelium. {ECO:0000269|PubMed:2527252, ECO:0000269|PubMed:2768245, ECO:0000269|PubMed:9063895}.
Post-translational modification
PTM: The secreted form is derived from the membrane-bound form either by cleavage of the GPI anchor, or/and by proteolysis catalyzed by a metalloprotease. {ECO:0000305|PubMed:3476960}.
Target Relevance information above includes information from UniProt accession : P15328
The UniProt Consortium

Data

ELISA with Human FOLR1 Protein
Immobilized Human FOLR1, His Tag at 1 µg/ml (100 µl/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA.
HPLC of Human FOLR1 Protein
The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human FOLR1 Protein
Human FOLR1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.