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Human FGF21 Protein 5211

$375.00$1,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: His29-Ser209
SKU: 5211parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9NSA1

express system

HEK293

product tag

N-mFc-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.

molecular weight

The protein has a predicted MW of 46.9 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human FGF21 Protein 5211

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Fibroblast growth factor 21 (FGF21) is a peptide hormone that is synthesized by several organs and regulates energy homeostasis. Excitement surrounding this relatively recently identified hormone is based on the documented metabolic beneficial effects of FGF21, which include weight loss and improved glycemia.
Protein names
Fibroblast growth factor 21 (FGF-21)
Gene names
FGF21,FGF21 UNQ3115/PRO10196
Protein family
Heparin-binding growth factors family
Mass
9606Da
Function
FUNCTION: Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB. Regulates systemic glucose homeostasis and insulin sensitivity. {ECO:0000250|UniProtKB:Q9JJN1, ECO:0000269|PubMed:15902306, ECO:0000269|PubMed:17623664}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9JJN1}.
Structure
SUBUNIT: Interacts (via C-terminus) with KLB; this interaction is direct. Interacts with FGFR4. {ECO:0000269|PubMed:15902306, ECO:0000269|PubMed:18829467, ECO:0000269|PubMed:19117008}.
Target Relevance information above includes information from UniProt accession : Q9NSA1
The UniProt Consortium

Data

SPR with Human FGF21 Protein
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
ELISA with Human FGF21 Protein
Immobilized Human FGF21 at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 2.29µg/ml determined by ELISA (QC Test).
HPLC of Human FGF21 Protein
The purity of Human FGF21 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human FGF21 Protein
Human FGF21 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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