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Human FGF basic Protein 4445

$68.00$225.00

Summary

  • Expression: E.coli
  • Active: Yes (cell based assay)
  • Amino Acid Range: Pro143-Ser288
SKU: 4445parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P09038

express system

E.coli

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE

background

FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

molecular weight

The protein has a predicted MW of 16.5 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.02 EU per μg by the LAL method.

Human FGF basic Protein 4445

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
Protein names
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Gene names
FGF2,FGF2 FGFB
Protein family
Heparin-binding growth factors family
Mass
9606Da
Function
FUNCTION: Acts as a ligand for FGFR1, FGFR2, FGFR3 and FGFR4 (PubMed:8663044). Also acts as an integrin ligand which is required for FGF2 signaling (PubMed:28302677). Binds to integrin ITGAV:ITGB3 (PubMed:28302677). Plays an important role in the regulation of cell survival, cell division, cell differentiation and cell migration (PubMed:28302677, PubMed:8663044). Functions as a potent mitogen in vitro (PubMed:1721615, PubMed:3732516, PubMed:3964259). Can induce angiogenesis (PubMed:23469107, PubMed:28302677). Mediates phosphorylation of ERK1/2 and thereby promotes retinal lens fiber differentiation (PubMed:29501879). {ECO:0000269|PubMed:1721615, ECO:0000269|PubMed:29501879, ECO:0000269|PubMed:3732516, ECO:0000269|PubMed:3964259}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:20230531}. Nucleus {ECO:0000269|PubMed:22321063}. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane (PubMed:20230531). Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol (PubMed:22321063). Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 (PubMed:22321063). {ECO:0000269|PubMed:20230531, ECO:0000269|PubMed:22321063}.
Tissues
TISSUE SPECIFICITY: Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. {ECO:0000269|PubMed:1417798, ECO:0000269|PubMed:1721615}.
Structure
SUBUNIT: Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2. Interacts with FGFBP3 (PubMed:18669637). Interacts with integrin ITGAV:ITGB3; the interaction is required for FGF2 signaling (PubMed:28302677). Interacts with SNORC (via the extracellular domain) (By similarity). Interacts with glypican GPC3 (By similarity). {ECO:0000250|UniProtKB:P13109, ECO:0000250|UniProtKB:P15655, ECO:0000269|PubMed:10358027, ECO:0000269|PubMed:11390973, ECO:0000269|PubMed:11509569, ECO:0000269|PubMed:11964394, ECO:0000269|PubMed:16257968, ECO:0000269|PubMed:18669637, ECO:0000269|PubMed:1885605, ECO:0000269|PubMed:20230531, ECO:0000269|PubMed:28302677, ECO:0000269|PubMed:8663044}.
Post-translational modification
PTM: Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. {ECO:0000269|PubMed:20230531}.; PTM: Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
Target Relevance information above includes information from UniProt accession : P09038
The UniProt Consortium

Data

ELISA with Human FGF basic Protein
Immobilized Human FGF basic at 0.5 µg/ml (100 µl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.6 ng/ml determined by ELISA (QC Test).
ELISA with Human FGF basic Protein
Immobilized Human FGF basic, No Tag at 5 µg/ml (100 µl/Well) on the plate. Dose response curve for Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.18 µg/ml determined by ELISA.
Cell based assay with Human FGF basic Protein
Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is 0.02 - 0.1 ng/ml (QC Test).
SDS-PAGE gel of Human FGF basic Protein
Human FGF basic on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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