Human Fc epsilon RI alpha/FCER1a Protein 3664

$203.00 – $675.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val26-Gln205

available size

Clear

SKU: 3664parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P12319
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
molecular weight	The protein has a predicted MW of 47.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Fc epsilon RI alpha/FCER1a Protein 3664

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Known susceptibility genes to atopy and asthma have been identified by linkage or associations with clinical phenotypes, including total serum IgE levels. IgE-mediated sensitivity reactions require a high-affinity IgE receptor (FcepsilonRI), which immobilizes the immunoglobulin on the surface of the effector cells, mostly mast cells and basophils. Similarly to the previously investigated beta subunit of the receptor, FCER1A is a good candidate for a quantitative trait locus (QTL) in allergic diseases, and appears to participate in the systemic regulation of IgE levels.
Protein names High affinity immunoglobulin epsilon receptor subunit alpha (Fc-epsilon RI-alpha) (FcERI) (IgE Fc receptor subunit alpha)
Protein family 348
Function High-affinity receptor for immunoglobulin epsilon/IgE. Mediates IgE effector functions in myeloid cells. Upon IgE binding and antigen/allergen cross-linking initiates signaling pathways that lead to myeloid cell activation and differentiation. On mast cells, basophils and eosinophils stimulates the secretion of vasoactive amines, lipid mediators and cytokines that contribute to inflammatory response, tissue remodeling and cytotoxicity against microbes. Triggers the immediate hypersensitivity response to allergens as a host defense mechanism against helminth parasites, pathogenic bacteria and venom toxicity. When dysregulated, it can elicit harmful life-threatening allergic and anaphylactic reactions.
Pathway 242
Subellular location Cell membrane ; Single-pass type I membrane protein.
Tissues Expressed in eosinophils.
Structure Tetramer of an alpha chain, a beta chain, and two disulfide linked gamma chains. Interacts with IGHE (via CH3 region).
Target Relevance information above includes information from UniProt accession: P12319
The UniProt Consortium

Data

ELISA with Human Fc epsilon RI alpha/FCER1a Protein

Immobilized Human Fc epsilon RI alpha, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-Fc epsilon RI alpha Antibody, hFc Tag with the EC50 14.5ng/ml determined by ELISA.

HPLC of Human Fc epsilon RI alpha/FCER1a Protein

The purity of Human Fc epsilon RI alpha is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Fc epsilon RI alpha/FCER1a Protein

Human Fc epsilon RI alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.