Human EPO/Erythropoietin Protein 2515

$180.00 – $600.00

Summary

Expression: HEK293
Active: Yes (cell based assay)
Amino Acid Range: Ala28-Arg193

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SKU: 2515parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	AAH93628
express system	HEK293
product tag	No Tag
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.
molecular weight	The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 0.1EU per μg by the LAL method.

Human EPO/Erythropoietin Protein 2515

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.
Protein names Erythropoietin (Epoetin)
Protein family EPO/TPO family
Mass 21307Da
Function Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed:28283061). Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3 (PubMed:9774108). {ECO:0000269\|PubMed:28283061, ECO:0000269\|PubMed:9774108}.
Subellular location Secreted {ECO:0000269\|PubMed:32989016}.
Tissues Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals. {ECO:0000269\|PubMed:3865178}.
Target Relevance information above includes information from UniProt accession: P01588
The UniProt Consortium

Data

ELISA with Human EPO/Erythropoietin~-Protein

Immobilized Human EPO, No Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.

Cell based assay with Human EPO/Erythropoietin~-Protein

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.4 -1 ng/mL (QC Test).

HPLC of Human EPO/Erythropoietin~-Protein

The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human EPO/Erythropoietin~-Protein

Human EPO on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.