Human E-Selectin/CD62E Protein 4615

$150.00 – $500.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Trp22-Pro556

SKU: 4615parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P16581
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 – 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
molecular weight	The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human E-Selectin/CD62E Protein 4615

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein names E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (ELAM-1) (Leukocyte-endothelial cell adhesion molecule 2) (LECAM2) (CD antigen CD62E)
Gene names SELE,SELE ELAM1
Protein family Selectin/LECAM family
Mass 9606Da
Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Catalytic activity BINDING 101..109; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 101; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:1ESL, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 103; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:1ESL, ECO:0007744\|PDB:1G1T, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 109; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 113..118; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 126..128; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 126; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:1ESL, ECO:0007744\|PDB:1G1T, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"; BINDING 127; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269\|PubMed:11081633, ECO:0000269\|PubMed:26117840, ECO:0007744\|PDB:1ESL, ECO:0007744\|PDB:1G1T, ECO:0007744\|PDB:4C16, ECO:0007744\|PDB:4CSY"
Subellular location Cell membrane ; Single-pass type I membrane protein.
Structure Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.
Target Relevance information above includes information from UniProt accession: P16581
The UniProt Consortium

Data

ELISA with Human E-Selectin/CD62E Protein

Immobilized Human E-Selectin, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA.

The purity of Human E-selectin is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human E-Selectin/CD62E Protein

Human E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.