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Human DNAM-1/CD226 Protein 4444

$203.00$675.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu19-Asn247
SKU: 4444parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q15762

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

molecular weight

The protein has a predicted MW of 28.9 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human DNAM-1/CD226 Protein 4444

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Protein names
CD226 antigen (DNAX accessory molecule 1) (DNAM-1) (CD antigen CD226)
Gene names
CD226,CD226 DNAM1
Mass
9606Da
Function
FUNCTION: Involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell (PubMed:8673704). Cell surface receptor for NECTIN2. Upon ligand binding, stimulates T-cell proliferation and cytokine production, including that of IL2, IL5, IL10, IL13, and IFNG. Competes with PVRIG for NECTIN2-binding (PubMed:26755705). {ECO:0000269|PubMed:26755705, ECO:0000269|PubMed:8673704}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I membrane protein {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Expressed by peripheral blood T-lymphocytes. {ECO:0000269|PubMed:8673704}.
Structure
SUBUNIT: Interacts with PVR and NECTIN2. Competes with PVRIG for NECTIN2-binding. {ECO:0000269|PubMed:15039383, ECO:0000269|PubMed:15607800, ECO:0000269|PubMed:26755705}.
Target Relevance information above includes information from UniProt accession : Q15762
The UniProt Consortium

Data

SPR with Human DNAM-1/CD226 Protein
Human CD155, hFc Tag captured on Protein A chip, can bind Human DNAM-1, His Tag with an affinity constant of 0.22µM as determined in a SPR assay (Biacore T200).
ELISA with Human DNAM-1/CD226 Protein
Immobilized Human DNAM-1, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 0.24µg/ml determined by ELISA (QC Test).
HPLC of Human DNAM-1/CD226 Protein
The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human DNAM-1/CD226 Protein
Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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