SPR with Human DNAM-1/CD226 Protein 4161

ELISA with Human DNAM-1/CD226 Protein 4161

Human DNAM-1/CD226 Protein 4161

$203.00 – $675.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu19-Asn247

SKU: 4161parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q15762
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
molecular weight	The protein has a predicted MW of 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human DNAM-1/CD226 Protein 4161

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Protein names CD226 antigen (DNAX accessory molecule 1) (DNAM-1) (CD antigen CD226)
Gene names CD226,CD226 DNAM1
Mass 38614Da
Function FUNCTION: Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells (PubMed:8673704, PubMed:9712030). Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells (PubMed:26755705, PubMed:31253644, PubMed:30591568). Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes (By similarity). {ECO:0000250\|UniProtKB:Q8K4F0, ECO:0000269\|PubMed:26755705, ECO:0000269\|PubMed:30591568, ECO:0000269\|PubMed:31253644, ECO:0000269\|PubMed:8673704, ECO:0000269\|PubMed:9712030}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269\|PubMed:15684041}; Single-pass type I membrane protein {ECO:0000305}. Note=Localizes to lipid rafts. {ECO:0000269\|PubMed:15684041}.
Tissues TISSUE SPECIFICITY: Expressed by peripheral blood T-lymphocytes. {ECO:0000269\|PubMed:8673704}.
Structure SUBUNIT: Interacts with PVR and NECTIN2 (PubMed:15039383, PubMed:15607800, PubMed:31253644, PubMed:30591568). Competes with PVRIG for NECTIN2-binding (PubMed:26755705). Interacts with ITGAL; this interaction mediates CD226 localization to lipid rafts (PubMed:15684041). {ECO:0000269\|PubMed:15039383, ECO:0000269\|PubMed:15607800, ECO:0000269\|PubMed:26755705, ECO:0000269\|PubMed:30591568, ECO:0000269\|PubMed:31253644}.
Post-translational modification PTM: PKC-mediated phosphorylation at Ser-329 is required for lipid raft recruitment (PubMed:15684041). Phosphorylation of Tyr-322 requires association with lipid rafts and allows GRB2 binding and activation of subsequent signaling (By similarity). {ECO:0000250\|UniProtKB:Q8K4F0, ECO:0000269\|PubMed:15684041}.
Target Relevance information above includes information from UniProt accession: Q15762
The UniProt Consortium

Data

Human DNAM-1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque CD155, His Tag with an affinity constant of 0.234 µM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human CD155, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 10.6ng/ml determined by ELISA (QC Test).

The purity of Human DNAM-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human DNAM-1/CD226 Protein

Human DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.