ELISA with Human/Cynomolgus Ephrin-A3/EFNA3 Protein 3828

Human/Cynomolgus Ephrin-A3/EFNA3 Protein 3828

$120.00 – $400.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln23-Ser213

SKU: 3828parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P52797
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.
molecular weight	The protein has a predicted MW of 41.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human/Cynomolgus Ephrin-A3/EFNA3 Protein 3828

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interaction of Eph receptor tyrosine kinases with their membrane bound ephrin ligands initiates bidirectional signaling events that regulate cell migratory and adhesive behavior. Whole-mount in situ hybridization revealed overlapping expression of the Epha1 receptor and its high-affinity ligands ephrin A1 (Efna1) and ephrin A3 (Efna3) in the primitive streak and the posterior paraxial mesoderm during early mouse development.
Protein names Ephrin-A3 (EFL-2) (EHK1 ligand) (EHK1-L) (EPH-related receptor tyrosine kinase ligand 3) (LERK-3)
Gene names EFNA3,EFNA3 EFL2 EPLG3 LERK3
Protein family Ephrin family
Mass 9606Da
Function Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling (By similarity).
Subellular location Cell membrane; Lipid-anchor, GPI-anchor.
Tissues Expressed in brain, skeletal muscle, spleen, thymus, prostate, testis, ovary, small intestine, and peripheral blood leukocytes.
Structure Interacts with EPHA8; activates EPHA8.
Target Relevance information above includes information from UniProt accession: P52797
The UniProt Consortium

Data

ELISA with Human/Cynomolgus Ephrin-A3/EFNA3 Protein

Immobilized Human EPHA10, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human/Cynomolgus EFNA3, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.

HPLC of Human/Cynomolgus Ephrin-A3/EFNA3 Protein

The purity of Human/Cynomolgus Ephrin-A3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human/Cynomolgus Ephrin-A3/EFNA3 Protein

Human/Cynomolgus Ephrin-A3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.