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Human CTLA-4/CD152 Protein 4443

$113.00$375.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Lys36-Asp161
SKU: 4443parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P16410

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.

molecular weight

The protein has a predicted MW of 14.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CTLA-4/CD152 Protein 4443

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Protein names
Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152)
Gene names
CTLA4,CTLA4 CD152
Mass
9606Da
Function
FUNCTION: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. {ECO:0000269|PubMed:16551244, ECO:0000269|PubMed:1714933}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:18468488, ECO:0000269|PubMed:28484017}; Single-pass type I membrane protein {ECO:0000269|PubMed:18468488, ECO:0000269|PubMed:28484017}. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalization.
Tissues
TISSUE SPECIFICITY: Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation. {ECO:0000269|PubMed:10493833, ECO:0000269|PubMed:16551244, ECO:0000269|PubMed:1713603}.
Structure
SUBUNIT: Homodimer; disulfide-linked (PubMed:11279501, PubMed:11279502, PubMed:21156796, PubMed:28484017, Ref.23). Binds to CD80/B7-1 and CD86/B7.2 (PubMed:11279501, PubMed:11279502, PubMed:28484017). Interacts with ICOSLG (PubMed:28484017). {ECO:0000269|PubMed:11279501, ECO:0000269|PubMed:11279502, ECO:0000269|PubMed:21156796, ECO:0000269|PubMed:28484017, ECO:0000269|Ref.23}.
Post-translational modification
PTM: N-glycosylation is important for dimerization. {ECO:0000269|PubMed:11279502, ECO:0000269|PubMed:16002699, ECO:0000269|PubMed:21156796}.; PTM: Phosphorylation at Tyr-201 prevents binding to the AP-2 adapter complex, blocks endocytosis, and leads to retention of CTLA4 on the cell surface. {ECO:0000269|PubMed:10842319, ECO:0000269|PubMed:9175836, ECO:0000269|PubMed:9813138}.
Target Relevance information above includes information from UniProt accession : P16410
The UniProt Consortium

Data

ELISA with Human CTLA-4/CD152 Protein
Immobilized Human CTLA-4, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test).
ELISA with Human CTLA-4/CD152 Protein
Immobilized Human CTLA-4, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
ELISA with Human CTLA-4/CD152 Protein
Immobilized Human CTLA-4, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
HPLC of Human CTLA-4/CD152 Protein
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CTLA-4/CD152 Protein
Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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