Human Coagulation factor XI Protein 3628

$345.00 – $1,150.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu19-Val625

available size

Clear

SKU: 3628parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P03951
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.
molecular weight	The protein has a predicted MW of 69.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human Coagulation factor XI Protein 3628

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Coagulation factor (F)XI has been described as a component of the early phase of the contact pathway of blood coagulation, acting downstream of factor XII.In contrast to its mild hemostatic activities, FXI is known to play a significant role in thrombosis, as it is a demonstrated independent risk factor for deep vein thrombosis, ischemic stroke, and myocardial infarction. A better understanding of the varied and complex role of FXI in both thrombosis and hemostasis will help to allow better prediction of bleeding risk in FXI-deficient patients and also informing the development of targeted agents to inhibit the thrombotic activities of FXI while preserving hemostasis.
Protein names Coagulation factor XI (FXI) (EC 3.4.21.27) (Plasma thromboplastin antecedent) (PTA) [Cleaved into: Coagulation factor XIa heavy chain; Coagulation factor XIa light chain]
Protein family Peptidase S1 family, Plasma kallikrein subfamily
Mass 70109Da
Function Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
Catalytic activity CATALYTIC ACTIVITY: Reaction=Selective cleavage of Arg-\|-Ala and Arg-\|-Val bonds in factor IX to form factor IXa.; EC=3.4.21.27;
Subellular location Secreted.
Tissues Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
Structure Homodimer; disulfide-linked. Forms a heterodimer with SERPINA5. After activation the heavy and light chains are also linked by a disulfide bond. Interacts with Anopheles gambiae D7L2 (PubMed:35460690). {ECO:0000269\|PubMed:18510371, ECO:0000269\|PubMed:1998667, ECO:0000269\|PubMed:35460690}.
Post-translational modification N-glycosylated on both chains. N-glycosylated sites mainly consist of nonfucosylated sialylated biantennary (in high abundance) and/or triantennary (in low abundance) complex structures. Glycosylation at Asn-163 uses a rare non-canonical Asn-X-Cys glycosite. {ECO:0000269\|PubMed:25092234}.; Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
Target Relevance information above includes information from UniProt accession: P03951
The UniProt Consortium

Data

ELISA with Human Coagulation factor XI Protein

Immobilized Human Coagulation factor XI, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Factor XI Antibody, hFc Tag with the EC50 of 7.4ng/ml determined by ELISA.

HPLC of Human Coagulation factor XI Protein

The purity of Human Coagulation factor XI is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Coagulation factor XI Protein

Human Coagulation factor XI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.