Human CD55 Protein 3496

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp35-Ser353

SKU: 3496parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P08174
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
molecular weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD55 Protein 3496

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
Protein names Complement decay-accelerating factor (CD antigen CD55)
Gene names CD55,CD55 CR DAF
Protein family Receptors of complement activation (RCA) family
Mass 9606Da
Function This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade (PubMed:7525274). Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage (PubMed:28657829).; (Microbial infection) Acts as a receptor for Coxsackievirus A21, coxsackieviruses B1, B3 and B5.; (Microbial infection) Acts as a receptor for Human enterovirus 70 and D68 (Probable).; (Microbial infection) Acts as a receptor for Human echoviruses 6, 7, 11, 12, 20 and 21.
Catalytic activity #N/A
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Lipid-anchor, GPI-anchor.; [Isoform 3]: Secreted .; [Isoform 4]: Secreted .; [Isoform 5]: Secreted .; [Isoform 6]: Cell membrane ; Lipid-anchor, GPI-anchor .; [Isoform 7]: Cell membrane ; Lipid-anchor, GPI-anchor .
Tissues Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.
Structure Monomer (major form) and non-disulfide-linked, covalent homodimer (minor form). Interacts with ADGRE5 (PubMed:11297558).; (Microbial infection) Interacts with coxsackievirus A21, coxsackieviruses B1, B3 and B5 capsid proteins.; (Microbial infection) Interacts with human enterovirus 70 and D68 capsid proteins (Probable).; (Microbial infection) Interacts with human echoviruses 6, 7, 11, 12, 20 and 21 capsid proteins.
Post-translational modification The Ser/Thr-rich domain is heavily O-glycosylated.
Domain Th
Target Relevance information above includes information from UniProt accession: P08174
The UniProt Consortium

Data

Immobilized Human CD55, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.

The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.

Human CD55 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.