Human CD5 Protein 4190

$218.00 – $725.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg25-Asn371

SKU: 4190parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P06127
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
molecular weight	The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD5 Protein 4190

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Protein names T-cell surface glycoprotein CD5 (Lymphocyte antigen T1/Leu-1) (CD antigen CD5)
Gene names CD5,CD5 LEU1
Mass 54578Da
Function FUNCTION: Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL (PubMed:1384049, PubMed:1385158). Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation (PubMed:23376399). Modulates B-cell biology through ERK1/2 activation in a Ca(2+)-dependent pathway via the non-selective Ca(2+) channel TRPC1, leading to IL-10 production (PubMed:27499044). {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1384049, ECO:0000269\|PubMed:1385158, ECO:0000269\|PubMed:23376399, ECO:0000269\|PubMed:27499044}.
Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000250\|UniProtKB:P13379}; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:P13379}.
Structure SUBUNIT: Interacts with CD72/LYB-2 (PubMed:1711157). Interacts with PTPN6/SHP-1 (By similarity). Interacts with CBL (PubMed:23376399). Interacts with CD5L (By similarity). Interacts with CD3Z/CD247 (PubMed:1384049, PubMed:1385158). {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1384049, ECO:0000269\|PubMed:1385158, ECO:0000269\|PubMed:1711157, ECO:0000269\|PubMed:23376399}.
Post-translational modification PTM: Phosphorylated on serine, threonine and tyrosine residues following TCR stimulation (PubMed:1385158). Phosphorylated by LCK on Tyr-453 and Tyr-487 upon TCR engagement. {ECO:0000250\|UniProtKB:P13379, ECO:0000269\|PubMed:1385158}.
Target Relevance information above includes information from UniProt accession: P06127
The UniProt Consortium

Data

Immobilized Human CD5 at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 14.6ng/ml determined by ELISA.

The purity of Human CD5 is greater than 95% as determined by SEC-HPLC.

Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.