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Human CD48/SLAMF2 Protein 4439

$255.00$850.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln27-Ser220
SKU: 4439parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P09326

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.

molecular weight

The protein has a predicted MW of 48.9 kDa. Due to glycosylation, the protein migrates to 62-72 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD48/SLAMF2 Protein 4439

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.
Protein names
CD48 antigen (B-lymphocyte activation marker BLAST-1) (BCM1 surface antigen) (Leukocyte antigen MEM-102) (SLAM family member 2) (SLAMF2) (Signaling lymphocytic activation molecule 2) (TCT.1) (CD antigen CD48)
Gene names
CD48,CD48 BCM1 BLAST1
Mass
9606Da
Function
FUNCTION: Glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein that interacts via its N-terminal immunoglobulin domain with cell surface receptors including 2B4/CD244 or CD2 to regulate immune cell function and activation (PubMed:12007789, PubMed:27249817). Participates in T-cell signaling transduction by associating with CD2 and efficiently bringing the Src family protein kinase LCK and LAT to the TCR/CD3 complex (PubMed:19494291). In turn, promotes LCK phosphorylation and subsequent activation (PubMed:12007789). Induces the phosphorylation of the cytoplasmic immunoreceptortyrosine switch motifs (ITSMs) of CD244 initiating a series of signaling events that leads to the generation of the immunological synapse and the directed release of cytolytic granules containing perforin and granzymes by T-lymphocytes and NK-cells (PubMed:27249817, PubMed:9841922). {ECO:0000269|PubMed:12007789, ECO:0000269|PubMed:19494291, ECO:0000269|PubMed:27249817, ECO:0000269|PubMed:9841922}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1999351, ECO:0000269|PubMed:9041467}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:1999351}. Secreted {ECO:0000269|PubMed:9418191}.
Tissues
TISSUE SPECIFICITY: Widely expressed on all hematopoietic cells. {ECO:0000269|PubMed:9893294}.
Structure
SUBUNIT: Interacts with CD2. Interacts with CD244 (PubMed:27249817, PubMed:9841922). Interacts with LCK (PubMed:12007789). {ECO:0000269|PubMed:12007789, ECO:0000269|PubMed:27249817, ECO:0000269|PubMed:9841922}.
Target Relevance information above includes information from UniProt accession : P09326
The UniProt Consortium

Data

ELISA with Human CD48/SLAMF2 Protein
Immobilized Human 2B4, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34µg/ml determined by ELISA.
HPLC of Human CD48/SLAMF2 Protein
The purity of Human CD48 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CD48/SLAMF2 Protein
Human CD48 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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