ELISA with Human CD40/TNFRSF5 Protein 4481

Human CD40/TNFRSF5 Protein 4481

$195.00 – $650.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu21-Arg193

SKU: 4481parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P25942
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
molecular weight	The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD40/TNFRSF5 Protein 4481

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Protein names Tumor necrosis factor receptor superfamily member 5 (B-cell surface antigen CD40) (Bp50) (CD40L receptor) (CDw40) (CD antigen CD40)
Gene names CD40,CD40 TNFRSF5
Mass 9606Da
Function Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).
Subellular location [Isoform I]: Cell membrane; Single-pass type I membrane protein.; [Isoform II]: Secreted.
Tissues B-cells and in primary carcinomas.
Structure Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation.
Target Relevance information above includes information from UniProt accession: P25942
The UniProt Consortium

Data

Immobilized Human CD40, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 79.7ng/ml determined by ELISA (QC Test).

Immobilized Human CD40, His Tag at 1µg/ml (100µl/Well). Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.

The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CD40/TNFRSF5 Protein

Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.