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Human CD40/TNFRSF5 Protein 4481

$195.00$650.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Glu21-Arg193
SKU: 4481parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P25942

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.

molecular weight

The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD40/TNFRSF5 Protein 4481

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Protein names
Tumor necrosis factor receptor superfamily member 5 (B-cell surface antigen CD40) (Bp50) (CD40L receptor) (CDw40) (CD antigen CD40)
Gene names
CD40,CD40 TNFRSF5
Mass
9606Da
Function
FUNCTION: Receptor for TNFSF5/CD40LG (PubMed:31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity). {ECO:0000250|UniProtKB:P27512, ECO:0000269|PubMed:31331973}.
Subellular location
SUBCELLULAR LOCATION: [Isoform I]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform II]: Secreted.
Tissues
TISSUE SPECIFICITY: B-cells and in primary carcinomas.
Structure
SUBUNIT: Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation. {ECO:0000269|PubMed:10984535, ECO:0000269|PubMed:12005438, ECO:0000269|PubMed:2463309, ECO:0000269|PubMed:7530216, ECO:0000269|PubMed:7533327, ECO:0000269|PubMed:9432981, ECO:0000269|PubMed:9511754, ECO:0000269|PubMed:9718306}.
Target Relevance information above includes information from UniProt accession : P25942
The UniProt Consortium

Data

ELISA with Human CD40/TNFRSF5 Protein
Immobilized Human CD40, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 79.7ng/ml determined by ELISA (QC Test).
ELISA with Human CD40/TNFRSF5 Protein
Immobilized Human CD40, His Tag at 1µg/ml (100µl/Well). Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.
HPLC of Human CD40/TNFRSF5 Protein
The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CD40/TNFRSF5 Protein
Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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