Human CD20 Protein-VLP 5139

$1,100.00 – $3,800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met1-Pro297

SKU: 5139parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P11836
express system	HEK293
product tag
purity	> 95% as determined by HPLC
background	B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R, CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
molecular weight	The target protein has a predicted MW of 34.3 kDa.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CD20 Protein-VLP 5139

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
Protein names
B-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD antigen CD20)
Gene names
MS4A1,MS4A1 CD20
Protein family
MS4A family
Mass
9606Da
Function
B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739).
Catalytic activity
#N/A
Subellular location
Cell membrane ; Multi-pass membrane protein. Cell membrane ; Lipid-anchor. Note=Constitutively associated with membrane rafts.
Tissues
Expressed on B-cells.
Structure
Forms homotetramers (PubMed:18474602, PubMed:7684739). Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR (PubMed:18474602).
Post-translational modification
Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate.
Target Relevance information above includes information from UniProt accession: P11836
The UniProt Consortium

Data

Immobilized Human CD20 VLP at 5µg/ml (100µl/Well) on plate on the plate. Dose response curve for Rituximab, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA (QC Test).

Immobilized Human CD20 VLP at 5µg/ml (100µl/Well) on plate on the plate. Dose response curve for Ofatumumab, hFc Tag with the EC50 of 10.5ng/ml determined by ELISA (QC Test).

Immobilized Human CD20 VLP at 5µg/ml (100µl/Well) on the plate. Dose response curve for Obinutuzumab, hFc Tag with the EC50 of 1.7ng/ml determined by ELISA.

The purity of Human CD20 VLP is greater than 95% as determined by SEC-HPLC.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.