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Human CD20/MS4A1 Protein 5181

$300.00$1,000.00

Summary

  • Expression: E.coli
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ile141-Ser188
SKU: 5181parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P11836

express system

E.coli

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE

background

B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R, CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.

molecular weight

The protein has a predicted MW of 16.8 kDa same as Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD20/MS4A1 Protein 5181

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R , CD117) and progressively increasing in concentration until maturity.CD20 is the target of the monoclonal antibodies rituximab, ocrelizumab, obinutuzumab, ofatumumab, ibritumomab tiuxetan, tositumomab, and ublituximab, which are all active agents in the treatment of all B cell lymphomas, leukemias, and B cell-mediated autoimmune diseases.
Protein names
B-lymphocyte antigen CD20 (B-lymphocyte surface antigen B1) (Bp35) (Leukocyte surface antigen Leu-16) (Membrane-spanning 4-domains subfamily A member 1) (CD antigen CD20)
Gene names
MS4A1,MS4A1 CD20
Protein family
MS4A family
Mass
9606Da
Function
FUNCTION: B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed:12920111, PubMed:3925015, PubMed:7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed:12920111, PubMed:18474602, PubMed:7684739). {ECO:0000269|PubMed:12920111, ECO:0000269|PubMed:18474602, ECO:0000269|PubMed:3925015, ECO:0000269|PubMed:7684739}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12920111, ECO:0000269|PubMed:22615937}; Multi-pass membrane protein {ECO:0000269|PubMed:22615937}. Cell membrane {ECO:0000269|PubMed:22615937}; Lipid-anchor {ECO:0000269|PubMed:22615937}. Note=Constitutively associated with membrane rafts. {ECO:0000269|PubMed:12920111}.
Tissues
TISSUE SPECIFICITY: Expressed on B-cells.
Structure
SUBUNIT: Forms homotetramers (PubMed:18474602, PubMed:7684739). Interacts with the heavy and light chains of cell surface IgM, the antigen-binding components of the BCR (PubMed:18474602). {ECO:0000269|PubMed:18474602, ECO:0000269|PubMed:7684739}.
Post-translational modification
PTM: Phosphorylated on serines and threonines in resting B-cells. Protein kinase C/PKC can use CD20 as substrate. {ECO:0000269|PubMed:2472394}.
Target Relevance information above includes information from UniProt accession : P11836
The UniProt Consortium

Data

ELISA with Human CD20/MS4A1 Protein
Immobilized Human CD20, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD20 Antibody, hFc Tag with the EC50 of 0.33µg/ml determined by ELISA.
SDS-PAGE gel of Human CD20/MS4A1 Protein
Human CD20 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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