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Human CD2/SRBC Protein 4850

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Lys25-Asp209
SKU: 4850parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P06729

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC

background

The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.

molecular weight

The protein has a predicted MW of 22.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD2/SRBC Protein 4850

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The CD2 family of receptors is evolutionarily conserved and widely expressed on cells within the hematopoietic compartment. In recent years several new members have been identified with important roles in the immune system. CD2 family members regulate natural killer (NK) cell lytic activity and inflammatory cytokine production when engaged by ligands on tumor cells.
Protein names
T-cell surface antigen CD2 (Erythrocyte receptor) (LFA-2) (LFA-3 receptor) (Rosette receptor) (T-cell surface antigen T11/Leu-5) (CD antigen CD2)
Gene names
CD2,CD2 SRBC
Mass
9606Da
Function
FUNCTION: CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23006327, ECO:0000269|PubMed:2437578}; Single-pass type I membrane protein {ECO:0000305}.
Tissues
TISSUE SPECIFICITY: Expressed in natural killer cells (at protein level). {ECO:0000269|PubMed:23006327}.
Structure
SUBUNIT: Interacts with CD48 (By similarity). Interacts with CD58 (LFA-3) (PubMed:10380930). Interacts with CD2AP (By similarity). Interacts with PSTPIP1 (PubMed:9857189). Interacts with FCGR3A; this interaction modulates NK cell activation and cytotoxicity. {ECO:0000250|UniProtKB:P08920, ECO:0000250|UniProtKB:P08921, ECO:0000269|PubMed:23006327, ECO:0000269|PubMed:9857189}.
Target Relevance information above includes information from UniProt accession : P06729
The UniProt Consortium

Data

SPR with Human CD2/SRBC Protein
Human CD2, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD58, hFc Tag with an affinity constant of 15.48 nM as determined in SPR assay (Biacore T200).
HPLC of Human CD2/SRBC Protein
The purity of Human CD2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human CD2/SRBC Protein
Human CD2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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