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Human CD19 Protein 4550

$375.00$1,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Pro20-Lys291
SKU: 4550parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P15391

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE

background

CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.

molecular weight

The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD19 Protein 4550

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Protein names
B-lymphocyte antigen CD19 (B-lymphocyte surface antigen B4) (Differentiation antigen CD19) (T-cell surface antigen Leu-12) (CD antigen CD19)
Gene names
CD19,CD19
Mass
9606Da
Function
FUNCTION: Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens (PubMed:1373518, PubMed:16672701, PubMed:2463100). Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores (PubMed:12387743, PubMed:16672701, PubMed:9317126, PubMed:9382888). Is not required for early steps during B cell differentiation in the blood marrow (PubMed:9317126). Required for normal differentiation of B-1 cells (By similarity). Required for normal B cell differentiation and proliferation in response to antigen challenges (PubMed:1373518, PubMed:2463100). Required for normal levels of serum immunoglobulins, and for production of high-affinity antibodies in response to antigen challenge (PubMed:12387743, PubMed:16672701, PubMed:9317126). {ECO:0000250|UniProtKB:P25918, ECO:0000269|PubMed:12387743, ECO:0000269|PubMed:1373518, ECO:0000269|PubMed:16672701, ECO:0000269|PubMed:2463100, ECO:0000269|PubMed:9317126, ECO:0000269|PubMed:9382888}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:1373518, ECO:0000269|PubMed:1383329, ECO:0000269|PubMed:16672701, ECO:0000269|PubMed:1702139, ECO:0000269|PubMed:2463100, ECO:0000269|PubMed:9317126, ECO:0000269|PubMed:9382888}; Single-pass type I membrane protein {ECO:0000305}. Membrane raft {ECO:0000250|UniProtKB:P25918}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P25918}.
Tissues
TISSUE SPECIFICITY: Detected on marginal zone and germinal center B cells in lymph nodes (PubMed:2463100). Detected on blood B cells (at protein level) (PubMed:16672701, PubMed:2463100). {ECO:0000269|PubMed:16672701, ECO:0000269|PubMed:2463100}.
Structure
SUBUNIT: Interacts with CR2/CD21 (PubMed:1702139). Part of a complex composed of CD19, CR2/CD21, CD81 and IFITM1/CD225 in the membrane of mature B-cells (PubMed:1383329). Interacts directly with CD81 (via the second extracellular domain); this interaction is initiated early during biosynthesis in the ER/pre-Golgi compartments and is essential for trafficking and compartmentalization of CD19 receptor on the cell surface of activated B cells (PubMed:16449649). Interacts with VAV (PubMed:10706702). Interacts with GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378. Interacts with PLCG2 when phosphorylated on Tyr-409 (PubMed:10706702). Interacts with LYN (PubMed:7687428). Interacts (when tyrosine phosphorylated) with the regulatory p85 subunit of phosphatidylinositol 3-kinase (PIK3R1 or PIK3R2) (PubMed:7684160). {ECO:0000269|PubMed:10706702, ECO:0000269|PubMed:1383329, ECO:0000269|PubMed:16449649, ECO:0000269|PubMed:1702139, ECO:0000269|PubMed:7684160, ECO:0000269|PubMed:7687428}.
Post-translational modification
PTM: Phosphorylated on tyrosine following B-cell activation (PubMed:10706702, PubMed:12387743, PubMed:7684160, PubMed:7687539). Phosphorylated on tyrosine residues by LYN (PubMed:7687428). Tyrosine residues are phosphorylated sequentially after activation of the B cell receptor. Phosphorylation of Tyr-531 is extremely rapid, followed by phosphorylation at Tyr-409. In contrast, phosphorylation of Tyr-500 appears more slowly and is more transient, returning rapidly to basal levels (By similarity). {ECO:0000250|UniProtKB:P25918, ECO:0000269|PubMed:10706702, ECO:0000269|PubMed:12387743, ECO:0000269|PubMed:7684160, ECO:0000269|PubMed:7687428, ECO:0000269|PubMed:7687539}.
Target Relevance information above includes information from UniProt accession : P15391
The UniProt Consortium

Data

ELISA with Human CD19 Protein
Immobilized Human CD19, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
ELISA with Human CD19 Protein
Immobilized Human CD19, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).
ELISA with Human CD19 Protein
Immobilized Human CD19, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
ELISA with Human CD19 Protein
Immobilized Human CD19, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
SDS-PAGE gel of Human CD19 Protein
Human CD19 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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