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Human CD155/PVR Protein 4441

$240.00$800.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Trp21-Asn343
SKU: 4441parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P15151

express system

HEK293

product tag

C-hFc

purity

> 95% as determined by Tris-Bis PAGE

background

CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor, " or PVR.

molecular weight

The protein has a predicted MW of 61.8 kDa. Due to glycosylation, the protein migrates to 75-100 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CD155/PVR Protein 4441

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
CD155 is a cell surface adhesion molecule functioning in tumor cell migration, invasion, and metastasis, and not surprisingly, is also designated as a common tumor-associated antigen. CD155 is also recognized by NK cells to induce their cytotoxicity. CD155 is also commonly referred to as the "poliovirus receptor," or PVR.
Protein names
Poliovirus receptor (Nectin-like protein 5) (NECL-5) (CD antigen CD155)
Gene names
PVR,PVR PVS
Protein family
Nectin family
Mass
9606Da
Function
FUNCTION: Mediates NK cell adhesion and triggers NK cell effector functions. Binds two different NK cell receptors: CD96 and CD226. These interactions accumulates at the cell-cell contact site, leading to the formation of a mature immunological synapse between NK cell and target cell. This may trigger adhesion and secretion of lytic granules and IFN-gamma and activate cytotoxicity of activated NK cells. May also promote NK cell-target cell modular exchange, and PVR transfer to the NK cell. This transfer is more important in some tumor cells expressing a lot of PVR, and may trigger fratricide NK cell activation, providing tumors with a mechanism of immunoevasion. Plays a role in mediating tumor cell invasion and migration. {ECO:0000269|PubMed:15471548, ECO:0000269|PubMed:15607800}.; FUNCTION: (Microbial infection) Acts as a receptor for poliovirus. May play a role in axonal transport of poliovirus, by targeting virion-PVR-containing endocytic vesicles to the microtubular network through interaction with DYNLT1. This interaction would drive the virus-containing vesicle to the axonal retrograde transport. {ECO:0000269|PubMed:2538245}.; FUNCTION: (Microbial infection) Acts as a receptor for Pseudorabies virus. {ECO:0000269|PubMed:9616127}.; FUNCTION: (Microbial infection) Is prevented to reach cell surface upon infection by Human cytomegalovirus /HHV-5, presumably to escape immune recognition of infected cell by NK cells. {ECO:0000269|PubMed:15640804}.
Subellular location
SUBCELLULAR LOCATION: [Isoform Alpha]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform Delta]: Cell membrane; Single-pass type I membrane protein.; SUBCELLULAR LOCATION: [Isoform Beta]: Secreted.; SUBCELLULAR LOCATION: [Isoform Gamma]: Secreted.
Structure
SUBUNIT: Can form trans-heterodimers with NECTIN3. The extracellular domain interacts with VTN, CD226 and CD96. The cytoplasmic domain interacts with DYNLT1. Binds with high affinity to TIGIT. {ECO:0000269|PubMed:11437656, ECO:0000269|PubMed:11751937, ECO:0000269|PubMed:12759359, ECO:0000269|PubMed:15034010, ECO:0000269|PubMed:15039383, ECO:0000269|PubMed:15194795, ECO:0000269|PubMed:16216929, ECO:0000269|PubMed:19011627, ECO:0000269|PubMed:22421438}.; SUBUNIT: (Microbial infection) Interacts with poliovirus capsid proteins. {ECO:0000269|PubMed:2538245}.; SUBUNIT: (Microbial infection) Interacts with human cytomegalovirus /HHV-5 UL141 protein. {ECO:0000269|PubMed:15640804}.; SUBUNIT: (Microbial infection) Interacts with pseudorabies virus gD protein. {ECO:0000269|PubMed:9616127}.
Post-translational modification
PTM: N-glycosylated. N-glycan at Asn-120: Hex5HexNAc4. {ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:22171320}.; PTM: Phosphorylated by Src kinases on tyrosine residues in the ITIM motif upon ligation. Interaction with TIGIT is required for Phosphorylation. {ECO:0000269|PubMed:15194502, ECO:0000269|PubMed:22421438}.
Domain
DOMAIN: Co
Target Relevance information above includes information from UniProt accession : P15151
The UniProt Consortium

Data

SPR with Human CD155/PVR Protein
Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 µM as determined in SPR assay (Biacore T200).
ELISA with Human CD155/PVR Protein
Immobilized Human TIGIT, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.1µg/ml determined by ELISA (QC Test).
ELISA with Human CD155/PVR Protein
Immobilized Human CD96, mFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human CD155, hFc Tag with the EC50 of 0.21µg/ml determined by ELISA.
SDS-PAGE gel of Human CD155/PVR Protein
Human CD155 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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