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Human CA125/MUC16 Protein 4581

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly12660-Met12923
SKU: 4581parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q8WXI7

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE

background

MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.

molecular weight

The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 50-80 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human CA125/MUC16 Protein 4581

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Protein names
Mucin-16 (MUC-16) (Ovarian cancer-related tumor marker CA125) (CA-125) (Ovarian carcinoma antigen CA125)
Gene names
MUC16,MUC16 CA125
Mass
1519175Da
Function
FUNCTION: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. {ECO:0000250}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Tissues
TISSUE SPECIFICITY: Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. {ECO:0000269|PubMed:11369781, ECO:0000269|PubMed:12218296, ECO:0000269|PubMed:16384952}.
Structure
SUBUNIT: Binds to MSLN. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Post-translational modification
PTM: Heavily O-glycosylated; expresses both type 1 and type 2 core glycans. {ECO:0000269|PubMed:12734200}.; PTM: Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans. {ECO:0000269|PubMed:12734200}.; PTM: May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. {ECO:0000269|PubMed:9276028}.; PTM: May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Domain
TOPO_DOM 1
Target Relevance information above includes information from UniProt accession : Q8WXI7
The UniProt Consortium

Data

ELISA with Human CA125/MUC16 Protein
Immobilized Human CA125, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 1.13µg/ml determined by ELISA.
SDS-PAGE gel of Human CA125/MUC16 Protein
Human CA125 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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