Human CA12/Carbonic anhydrase XII Protein 2776

$270.00 – $900.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Ala25-Ser301

available size

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SKU: 2776parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	O43570
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development.
molecular weight	The protein has a predicted MW of 32.22 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human CA12/Carbonic anhydrase XII Protein 2776

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Carbonic anhydrases (CAs) are a family of enzymes involved in the pH regulation of metabolically active cells/tissues. Carbonic anhydrase XII (CA XII) is a key mediator of several signaling pathways that are involved in cancer development.
Protein names Carbonic anhydrase 12 (EC 4.2.1.1) (Carbonate dehydratase XII) (Carbonic anhydrase XII) (CA-XII) (Tumor antigen HOM-RCC-3.1.3)
Gene names CA12,CA12
Protein family Alpha-carbonic anhydrase family
Mass 9606Da
Function Reversible hydration of carbon dioxide.
Catalytic activity #N/A
Subellular location Membrane; Single-pass type I membrane protein. Cell membrane .
Tissues Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Expressed in sweat glands and bronchiolar epithelium (PubMed:26911677).
Structure Homodimer.
Target Relevance information above includes information from UniProt accession: O43570
The UniProt Consortium

Data

Activity assay with Human CA12/Carbonic anhydrase XII Protein

Measured by its esterase activity. The specific activity is > 40 pmol/min/µg.

HPLC of Human CA12/Carbonic anhydrase XII Protein

The purity of Human CA12 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human CA12/Carbonic anhydrase XII Protein

Human CA12 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.