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Human Beta Klotho Protein 4670

$315.00$1,050.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met30-Thr983
SKU: 4670parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q86Z14

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.

molecular weight

The protein has a predicted MW of 111.6 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human Beta Klotho Protein 4670

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Beta-klotho (KLB) is a coreceptor required for endocrine fibroblast growth factor (FGF) 15/19 and FGF21 signaling in the brain. Klb is prominent within the hypothalamus, which is consistent with its metabolic functions, but diverse roles for Klb are now emerging. Central Klb expression is low but discrete and may govern FGF-targeted sites.
Protein names
Beta-klotho (BKL) (BetaKlotho) (Klotho beta-like protein)
Gene names
KLB,KLB
Protein family
Glycosyl hydrolase 1 family, Klotho subfamily
Mass
9606Da
Function
FUNCTION: Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity). {ECO:0000250}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type III membrane protein {ECO:0000305}.
Structure
SUBUNIT: Interacts with FGFR1 and FGFR4 (By similarity). Interacts with FGF19; this interaction is direct. Interacts (via C-terminus) with FGF21; this interaction is direct. {ECO:0000250, ECO:0000269|PubMed:18829467, ECO:0000269|PubMed:19117008}.
Domain
DOMAIN: Co
Target Relevance information above includes information from UniProt accession : Q86Z14
The UniProt Consortium

Data

SPR with Human Beta Klotho Protein
Human Beta Klotho, His Tag captured on CM5 Chip via anti-His antibody can bind Human FGF21, mFc Tag with an affinity constant of 0.54nM as determined in SPR assay (Biacore T200).
ELISA with Human Beta Klotho Protein
Immobilized Human Beta Klotho at 2µg/ml (100µl/Well). Dose response curve for Anti-Beta Klotho Antibody1, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA (QC Test).
ELISA with Human Beta Klotho Protein
Immobilized Human Beta Klotho at 2µg/ml (100µl/Well). Dose response curve for Anti-Beta Klotho Antibody2, hFc Tag with the EC50 of 34.6ng/ml determined by ELISA (QC Test).
ELISA with Human Beta Klotho Protein
Immobilized Human FGF21, mFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Human Beta Klotho, His Tag with the EC50 of 1.48µg/ml determined by ELISA.
HPLC of Human Beta Klotho Protein
The purity of Human Beta Klotho is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human Beta Klotho Protein
Human Beta Klotho on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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