SPR with Human BAFF/TNFSF13B/CD257 Protein 4418

ELISA with Human BAFF/TNFSF13B/CD257 Protein 4418

Human BAFF/TNFSF13B/CD257 Protein 4418

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala134-Leu285

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SKU: 4418parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q9Y275
express system	HEK293
product tag	N-monomeric hFc
purity	> 90% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
molecular weight	The protein has a predicted MW of 41.7 kDa. Due to glycosylation, the protein migrates to 45-48 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human BAFF/TNFSF13B/CD257 Protein 4418

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
B-cell activating factor (BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene.BAFF is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFF-R.
Protein names Tumor necrosis factor ligand superfamily member 13B (B lymphocyte stimulator) (BLyS) (B-cell-activating factor) (BAFF) (Dendritic cell-derived TNF-like molecule) (TNF- and APOL-related leukocyte expressed ligand 1) (TALL-1) (CD antigen CD257) [Cleaved into: Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form]
Gene names TNFSF13B,TNFSF13B BAFF BLYS TALL1 TNFSF20 ZTNF4 UNQ401/PRO738
Protein family Tumor necrosis factor family
Mass 31223Da
Function FUNCTION: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. {ECO:0000269\|PubMed:10973284}.; FUNCTION: Isoform 2 seems to inhibit isoform 1 secretion and bioactivity. {ECO:0000250}.; FUNCTION: [Isoform 3]: Acts as a transcription factor for its own parent gene, in association with NF-kappa-B p50 subunit, at least in autoimmune and proliferative B-cell diseases. The presence of Delta4BAFF is essential for soluble BAFF release by IFNG/IFN-gamma-stimulated monocytes and for B-cell survival. It can directly or indirectly regulate the differential expression of a large number of genes involved in the innate immune response and the regulation of apoptosis. {ECO:0000269\|PubMed:10973284}.
Subellular location SUBCELLULAR LOCATION: Cell membrane; Single-pass type II membrane protein.; SUBCELLULAR LOCATION: [Tumor necrosis factor ligand superfamily member 13b, soluble form]: Secreted.
Tissues TISSUE SPECIFICITY: Abundantly expressed in peripheral blood Leukocytes and is specifically expressed in monocytes and macrophages. Also found in the spleen, lymph node, bone marrow, T-cells and dendritic cells. A lower expression seen in placenta, heart, lung, fetal liver, thymus, and pancreas. Isoform 2 is expressed in many myeloid cell lines. {ECO:0000269\|PubMed:12867412}.
Structure SUBUNIT: Homotrimer. Isoform 2 heteromultimerizes with isoform 1, probably limiting the amount of functional isoform 1 on the cell surface. Isoform 3 is unlikely form trimers or bind to BAFF receptors. {ECO:0000269\|PubMed:12867412, ECO:0000269\|PubMed:22749832}.
Post-translational modification PTM: The soluble form derives from the membrane form by proteolytic processing.; PTM: Isoform 2 is not efficiently shed from the membrane unlike isoform 1. {ECO:0000250}.; PTM: N-glycosylated.
Target Relevance information above includes information from UniProt accession: Q9Y275
The UniProt Consortium

Data

SPR with Human BAFF/TNFSF13B/CD257 Protein

Human BAFF, hFc Tag captured on CM5 Chip via Protein A can bind Mouse BCMA, His-Avi Tag with an affinity constant of 5.83 µM as determined in SPR assay (Biacore T200).

ELISA with Human BAFF/TNFSF13B/CD257 Protein

Immobilized Human BCMA Trimer, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human BAFF, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA (QC Test).

HPLC of Human BAFF/TNFSF13B/CD257 Protein

The purity of Human BAFF is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human BAFF/TNFSF13B/CD257 Protein

Human BAFF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.