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Human B7-H6/NCR3LG1 Protein 4417

$270.00$900.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Asp25-Ser262
SKU: 4417parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q68D85

express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.

molecular weight

The protein has a predicted MW of 27.8 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human B7-H6/NCR3LG1 Protein 4417

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
B7-H6 is a glycosylated member of the B7 family of immune co‑stimulatory proteins. which is a ligand for the NK cell activating receptor NKp30, was targeted to create a CAR that targets multiple tumor types. B7H6 is expressed on various primary human tumors, including leukemia, lymphoma and gastrointestinal stromal tumors, but it is not constitutively expressed on normal tissues.
Protein names
Natural cytotoxicity triggering receptor 3 ligand 1 (B7 homolog 6) (B7-H6)
Gene names
NCR3LG1,NCR3LG1 B7H6
Mass
9606Da
Function
FUNCTION: Triggers NCR3-dependent natural killer cell activation. {ECO:0000269|PubMed:19528259}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:19528259}; Single-pass type I membrane protein {ECO:0000269|PubMed:19528259}.
Tissues
TISSUE SPECIFICITY: Not detected in any normal tissue tested. Expressed at the surface of several tumor cell lines including T and B-lymphomas, myeloid leukemias, melanomas, carcinomas and large T SV40 antigen-transformed cells (at protein level). {ECO:0000269|PubMed:19528259}.
Structure
SUBUNIT: Monomer. Interacts specifically with NCR3, but not with other natural killer cell-activating receptors, including NCR1, NCR2 and KLRK1. {ECO:0000269|PubMed:19528259, ECO:0000269|PubMed:21422170}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : Q68D85
The UniProt Consortium

Data

SPR with Human B7-H6/NCR3LG1 Protein
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10nM as determined in SPR assay (Biacore T200).
HPLC of Human B7-H6/NCR3LG1 Protein
The purity of Human B7-H6 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human B7-H6/NCR3LG1 Protein
Human B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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