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Human B7-1/CD80 Protein 4545

$225.00$750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Val35-Asn242
SKU: 4545parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P33681

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.

molecular weight

The protein has a predicted MW of 26.8 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Human B7-1/CD80 Protein 4545

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Protein names
T-lymphocyte activation antigen CD80 (Activation B7-1 antigen) (BB1) (CTLA-4 counter-receptor B7.1) (B7) (CD antigen CD80)
Gene names
CD80,CD80 CD28LG CD28LG1 LAB7
Mass
9606Da
Function
FUNCTION: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. {ECO:0000269|PubMed:10583602}.; FUNCTION: (Microbial infection) Acts as a receptor for adenovirus subgroup B. {ECO:0000269|PubMed:16920215}.
Subellular location
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
Tissues
TISSUE SPECIFICITY: Expressed on activated B-cells, macrophages and dendritic cells.
Structure
SUBUNIT: Homodimer; CD80 dimers on the antigen presenting cells (APCs) bridge CTLA4/CD152 dimers on T-cells in a periodic zipper-like arrangement. {ECO:0000269|PubMed:10583602, ECO:0000269|PubMed:11279502}.; SUBUNIT: (Microbial infection) Interacts with adenovirus subgroup B fiber proteins. {ECO:0000269|PubMed:16920215}.; SUBUNIT: (Microbial infection) Interacts with Orthopoxvirus OPG038/M2 protein, inhibiting the interaction with CTLA4/CD152. {ECO:0000269|PubMed:30918073}.
Target Relevance information above includes information from UniProt accession : P33681
The UniProt Consortium

Data

SPR with Human B7-1/CD80 Protein
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
ELISA with Human B7-1/CD80 Protein
Immobilized Human CTLA-4, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human B7-1, His Tag with the EC50 of 1.48µg/ml determined by ELISA (QC Test).
HPLC of Human B7-1/CD80 Protein
The purity of Human B7-1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Human B7-1/CD80 Protein
Human B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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