Human Alkaline Phosphatase (Placental type) /ALPP Protein 3477

$300.00 – $1,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile23-Asp506

available size

Clear

SKU: 3477parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P05187
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
molecular weight	The protein has a predicted MW of 53.8 kDa. Due to glycosylation, the protein migrates to 63-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Human Alkaline Phosphatase (Placental type) /ALPP Protein 3477

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease.
Protein names Alkaline phosphatase, placental type (EC 3.1.3.1) (Alkaline phosphatase Regan isozyme) (Placental alkaline phosphatase 1) (PLAP-1)
Gene names ALPP,ALPP PLAP
Protein family Alkaline phosphatase family
Mass 9606Da
Function Alkaline phosphatase that can hydrolyze various phosphate compounds.
Catalytic activity BINDING 64; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 64; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 114; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 177; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 238; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 291; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 292; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 307; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250\|UniProtKB:P05186"; BINDING 333; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 338; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 342; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 379; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 380; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"; BINDING 454; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000269\|PubMed:11124260, ECO:0000269\|PubMed:15946677, ECO:0000269\|PubMed:16815919, ECO:0000269\|PubMed:20693656"
Subellular location Cell membrane; Lipid-anchor, GPI-anchor .
Tissues Detected in placenta (at protein level).
Structure Homodimer.
Target Relevance information above includes information from UniProt accession: P05187
The UniProt Consortium

Data

ELISA with Human Alkaline Phosphatase (Placental type) /ALPP Protein

Immobilized Human Alkaline Phosphatase (Placental type) , His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 25.7ng/ml determined by ELISA.

HPLC of Human Alkaline Phosphatase (Placental type) /ALPP Protein

The purity of Human Alkaline Phosphatase (Placental type) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Human Alkaline Phosphatase (Placental type) /ALPP Prote

Human Alkaline Phosphatase (Placental type) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.