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Human Activin A Protein 2944

$225.00$750.00

Summary

  • Expression: HEK293
  • Active: Yes (cell based assay)
  • Amino Acid Range: Gly311-Ser426
SKU: 2944parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

P08476

express system

HEK293

product tag

No Tag

purity

> 95% as determined by Tris-Bis PAGE

background

Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.

molecular weight

The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 0.1EU per μg by the LAL method.

Human Activin A Protein 2944

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Activin-A, a member of the TGF-β superfamily, Extensive research over the past decades illuminated fundamental roles for activin-A in essential biologic processes, including embryonic development, stem cell maintenance and differentiation, haematopoiesis, cell proliferation and tissue fibrosis. Activin-A signals through two type I and two type II receptors which, upon ligand binding, activate their kinase activity, phosphorylate the SMAD2 and 3 intracellular signaling mediators that form a complex with SMAD4, translocate to the nucleus and activate or silence gene expression.
Protein names
Inhibin beta A chain (Activin beta-A chain) (Erythroid differentiation protein) (EDF)
Gene names
INHBA,INHBA
Protein family
TGF-beta family
Mass
9606Da
Function
Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Subellular location
Secreted.
Structure
Dimeric, linked by one or more disulfide bonds. Inhibin A is a dimer of alpha and beta-A. Inhibin B is a dimer of alpha and beta-B. Activin A is a homodimer of beta-A. Activin B is a homodimer of beta-B. Activin AB is a dimer of beta-A and beta-B. Interacts with FST and FSTL3.
Target Relevance information above includes information from UniProt accession: P08476
The UniProt Consortium

Data

ELISA with Human Activin A Protein
Immobilized Human Activin A, No Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human Activin RIIB, His Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).
ELISA with Human Activin A Protein
Immobilized Human Activin A, No Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human Activin RIIA, His Tag with the EC50 of 0.23µg/ml determined by ELISA.
Cell based assay with Human Activin A Protein
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL (QC Test).
SDS-PAGE gel of Human Activin A Protein
Human Activin A on Tris-Bis PAGE under reduced (R) condition and Non reducing (N) condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information.

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