Human ABO Protein 2346

$300.00 – $1,000.00

Summary

Expression: HEK293
Pure: Yes (SDS-PAGE)
Amino Acid Range: Ala54-Pro354

SKU: 2346parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	ADX99264
express system	HEK293
product tag	N-hFc
purity	> 95% as determined by Tris-Bis PAGE
background	Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants which represent secondary gene products. They are synthesized in a stepwise fashion from a precursor by the action of different glycosyltransferases.
molecular weight	The protein has a predicted MW of 60.56 kDa. Due to glycosylation, the protein migrates to 62-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Human ABO Protein 2346

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Histo-blood group ABH (O) antigens are major alloantigens in humans. These antigens are widely distributed in human tissues and undergo changes in expression during cellular differentiation and malignant development. The ABH antigens have been characterized as terminal disaccharide determinants which represent secondary gene products. They are synthesized in a stepwise fashion from a precursor by the action of different glycosyltransferases.
Protein names Histo-blood group ABO system transferase (Fucosylglycoprotein 3-alpha-galactosyltransferase) (Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase) (Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase) (EC 2.4.1.40) (Glycoprotein-fucosylgalactoside alpha-galactosyltransferase) (EC 2.4.1.37) (Histo-blood group A
Protein family Glycosyltransferase 6 family
Mass 40934Da
Function This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.; Glycosyltransferase that catalyzes the transfer of carbohydrates to H antigen, forming the antigenic structures of the ABO blood group. {ECO:0000269\|PubMed:2121736}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-N-acetyl-alpha-D-galactosamine = an N-acetyl-alpha-D-galactosaminyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D-galactosyl derivative + H(+) + UDP; Xref=Rhea:RHEA:19021, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:67138, ChEBI:CHEBI:140327, ChEBI:CHEBI:140559; EC=2.4.1.40; Evidence={ECO:0000269\|PubMed:12198488, ECO:0000269\|PubMed:17259183}; CATALYTIC ACTIVITY: Reaction=an alpha-L-fucosyl-(1->2)-beta-D-galactosyl derivative + UDP-alpha-D-galactose = an alpha-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->2)]-beta-D-galactosyl derivative + H(+) + UDP; Xref=Rhea:RHEA:14349, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:66914, ChEBI:CHEBI:140327, ChEBI:CHEBI:140328; EC=2.4.1.37; Evidence={ECO:0000269\|PubMed:12198488, ECO:0000269\|PubMed:17259183};
Pathway PATHWAY: Protein modification; protein glycosylation. {ECO:0000305}.
Subellular location Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein {ECO:0000269\|PubMed:18513251}. Secreted. Note=Membrane-bound form in trans cisternae of Golgi. Secreted into the body fluid.
Tissues Expressed at high levels in testis. Also expressed in pancreas, uterus and lung and salivary gland. {ECO:0000269\|PubMed:7598760}.
Post-translational modification The soluble form derives from the membrane form by proteolytic processing.
Domain The conserved DXD motif is involved in cofactor binding. The manganese ion interacts with th
Target Relevance information above includes information from UniProt accession: P16442
The UniProt Consortium

Data

Human ABO on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.