| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | VSV-G |
| available sizes | 1 mg |
goat anti-VSV-G HRP polyclonal antibody 1734
$3,142.00
Antibody summary
- Goat polyclonal to VSV-G , HRP conjugate
- Suitable for: ELISA,ICC,IHC,WB
- Isotype: Whole IgG
- 1 mg
goat anti-VSV-G HRP polyclonal antibody 1734
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen Synthetic peptide YTDIEMNRLGK conjugated to KLH. |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.09 |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| VSV-G tag |
| Protein names VSV-G epitope tag |
| Alternative names VSV-G tag, VSV epitope, Vesicular stomatitis virus glycoprotein tag, YTDIEMNRLGK tag |
| Function The VSV-G tag is an artificial peptide epitope used for detection and characterization of recombinant proteins using anti-VSV-G antibodies. |
| Description The VSV-G tag is a peptide epitope derived from the glycoprotein G of Vesicular stomatitis virus (VSV). It is commonly used as a recombinant protein tag for detection, localization, and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Immunoprecipitation, Protein localization, Recombinant protein |
| Sequence YTDIEMNRLGK |
| Structure The VSV-G tag is an 11-amino acid peptide epitope with the sequence YTDIEMNRLGK derived from the vesicular stomatitis virus glycoprotein G. |
| Function The VSV-G tag is used as a molecular epitope tag for detection, immunoprecipitation, purification, and localization of recombinant proteins. |
| Detection VSV-G-tagged proteins can be detected using anti-VSV-G monoclonal antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, flow cytometry, and immunoprecipitation assays. |
| Applications The VSV-G tag is typically fused to recombinant proteins at the N- or C-terminus to facilitate detection while minimizing effects on protein function. |
| Research relevance The VSV-G tag is a well-established recombinant protein tag and is commonly used alongside FLAG, HA, Myc, V5, and His tags in molecular biology and biotechnology. |
Data
 |
| Detection of VSV-G-tagged fusion protein in 200, 100, and 50ng of E. coli lysate with #1734 diluted 1:25,000. |
FAQ & Publications
Frequently Asked Questions
What applications has the goat anti-VSV-G HRP polyclonal antibody 1734 been validated for?
This antibody has been tested and validated for use in Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA assays.
What are the recommended dilutions for using this antibody in ELISA and immunoblotting?
For ELISA, the recommended dilution range is 1:10,000 to 1:100,000. For immunoblotting (Western blot), use a dilution range of 1:1,000 to 1:30,000. Optimal dilutions should be determined by the end user for specific applications.
How should the goat anti-VSV-G HRP polyclonal antibody be stored to maintain stability?
The antibody should be stored in liquid form at 2-8°C. It is stable for at least one year under these conditions.
What is the host species and clonality of this anti-VSV-G antibody?
The antibody is a goat polyclonal IgG, generated against the VSV-G epitope.
What immunogen was used to produce this goat anti-VSV-G antibody?
The immunogen is a synthetic peptide with the sequence YTDIEMNRLGK conjugated to keyhole limpet hemocyanin (KLH).
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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