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goat anti-VSV-G HRP polyclonal antibody 1734

$3,142.00

Antibody summary

  • Goat polyclonal to VSV-G , HRP conjugate
  • Suitable for: ELISA,ICC,IHC,WB
  • Isotype: Whole IgG
  • 1 mg
SKU: 1734parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

goat

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

VSV-G

available sizes

1 mg

goat anti-VSV-G HRP polyclonal antibody 1734

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Immunogen
Synthetic peptide YTDIEMNRLGK conjugated to KLH.
Size and concentration
1mg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
Phosphate Buffered Saline (PBS) containing 0.09
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689
donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control
Goat polyclonal - Isotype Control
target relevance
Biotechnology
The VSV-G tag has become a valuable and versatile tool in scientific research, particularly in molecular biology, cell biology, and virology studies. The VSV-G tag is derived from the glycoprotein of the Vesicular Stomatitis Virus (VSV), a virus that infects animals and does not replicate in mammalian cells. This feature makes the VSV-G tag highly useful for pseudotyping viral particles and creating viral vectors for gene delivery and expression studies. Researchers frequently use the VSV-G tag to replace the native envelope protein of viral vectors, enabling efficient and broad transduction of target cells, including difficult-to-transfect cell types. Furthermore, the VSV-G tag is often employed as a fusion tag for protein expression and purification, as it is well-tolerated and effectively trafficked to the plasma membrane. The VSV-G tag can be readily detected using specific antibodies in a variety of experimental assays, such as Western blotting and immunofluorescence, facilitating the visualization and quantification of tagged proteins. The widespread application of the VSV-G tag in research can be attributed to its utility in viral vector design, as well as its compatibility with multiple expression systems, making it a valuable tool for investigating cellular processes, gene function, and protein dynamics in diverse biological contexts.

Data

benchmark-antibodies_anti-vsv-g_antibody_hrp_conjugate_1734_1.jpg
Detection of VSV-G-tagged fusion protein in 200, 100, and 50ng of E. coli lysate with #1734 diluted 1:25,000.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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