| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA, ICC/IF, WB |
| available sizes | 100 µg |
goat anti-V5 tag polyclonal antibody 6568
$426.00
Antibody summary
- Goat polyclonal to V5 tag
- Suitable for: WB, ICC/IF, ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgG
- 100 µg
goat anti-V5 tag polyclonal antibody 6568
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500 These are |
| Immunogen Synthetic peptide corresponding to aa 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5 conjugated to KLH. |
| Size and concentration 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| V5 tag |
| Protein names V5 epitope tag |
| Alternative names V5 tag, V5 epitope, SV5 tag, Pk tag |
| Function The V5 tag is an artificial peptide epitope used for the detection, purification, and characterization of recombinant proteins. It is recognized by anti-V5 antibodies in applications including western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Description The V5 tag is a synthetic peptide epitope derived from the P and V proteins of Human orthorubulavirus 5 (formerly Simian Virus 5, SV5). It is commonly fused to recombinant proteins for detection, purification, and localization studies. |
| Keywords Epitope tag, Protein detection, Protein purification, Recombinant protein |
| Sequence GKPIPNPLLGLDST |
| Structure The V5 tag is a 14-amino acid peptide epitope with the sequence GKPIPNPLLGLDST derived from the P and V proteins of Human orthorubulavirus 5 (formerly SV5). |
| Function The V5 tag is used as a molecular tag for the detection, purification, and localization of recombinant proteins. |
| Applications V5-tagged proteins can be detected by western blotting, ELISA, immunofluorescence, immunohistochemistry, flow cytometry, and immunoprecipitation. |
| Research relevance The V5 tag is widely used in recombinant protein expression systems and serves as an alternative to FLAG, HA, Myc, and His tags. |
Data
 |
| Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody 6568 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
|
| Detection of V5-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-V5 antibody 6568 used for WB at 0.04 µg /ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
FAQ & Publications
Frequently Asked Questions
What applications has the goat anti-V5 tag polyclonal antibody 6568 been tested for?
This antibody has been tested and is suitable for Western blotting (WB), immunocytochemistry/immunofluorescence (ICC/IF), enzyme-linked immunosorbent assay (ELISA), and immunoprecipitation (IP).
How should the goat anti-V5 tag polyclonal antibody 6568 be stored to maintain stability?
The antibody is stable for at least one year when stored at 2-8°C in its supplied storage buffer, which is PBS, pH 7.2, containing 0.09% sodium azide (NaN3).
What is the host species and clonality of the anti-V5 tag antibody 6568?
This antibody is a goat polyclonal antibody with an IgG isotype, generated against the V5 epitope tag peptide conjugated to KLH as the immunogen.
Which secondary antibodies are compatible with the goat anti-V5 tag polyclonal antibody 6568 for detection?
Compatible secondary antibodies include donkey anti-goat IgG, H&L chain specific polyclonal antibodies conjugated to peroxidase, biotin, or FITC, including cross-absorbed versions, such as catalog numbers 1689, 1699, 1704, 1705, and 1709.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
Only logged in customers who have purchased this product may leave a review.
Reviews
There are no reviews yet.