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goat anti-T7 HRP polyclonal antibody 5237

$3,142.00

Antibody summary

  • Goat polyclonal to T7 , HRP conjugate
  • Suitable for: WB,IHC,ICC,ELISA
  • Isotype: Whole IgG
  • 1 mg
SKU: 5237parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

goat

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

T7

available sizes

1 mg

Available product – goat anti-T7 HRP polyclonal antibody 5237

antibody
Tested applications
WB,IHC,IHC,ICC/IF,ELISA
Recommended dilutions
Immunoblotting: use at a dilution of 1:1,000- 1:30,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

ELISA: use at a dilution of 1:10,000- 1:100,000

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Immunogen
T7 (MASMTGGQQMG) conjugated to KLH.
Size and concentration
1mg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at 2-8°C.
Storage buffer
Phosphate Buffered Saline (PBS) containing 0.2%
Purity
peptide affinty purifcation
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689
donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control
Goat polyclonal - Isotype Control
target relevance
Biotechnology
The T7 tag has emerged as a valuable tool in scientific research, particularly in molecular biology and protein expression studies. The T7 tag is a short peptide sequence (MASMTGGQQMG) derived from the T7 bacteriophage gene 10, which can be fused to the N or C terminus of a target protein. Its small size and minimal impact on the function of the tagged protein make it an attractive choice as a molecular tag. Researchers frequently use the T7 tag in protein expression systems, such as bacterial, insect, or mammalian cells, to facilitate the identification and purification of the recombinant protein. The T7 tag’s versatility allows for straightforward detection using specific T7 tag antibodies in various experimental assays, including Western blotting and immunoprecipitation. Moreover, the T7 tag can be employed for protein purification through affinity chromatography using T7 tag-specific resins, simplifying downstream characterization and functional studies. The widespread adoption of the T7 tag in research owes to its efficiency, reproducibility, and compatibility with a range of expression systems, making it an indispensable tool for investigating protein interactions, post-translational modifications, and protein localization in diverse biological contexts.

Data

benchmark-antibodies_anti-t7_antibody_hrp_conjugate_5237_1.jpg
Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-T7 antibody 5237 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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