| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | T7 |
| available sizes | 1 mg |
goat anti-T7 HRP polyclonal antibody 5237
$3,142.00
Antibody summary
- Goat polyclonal to T7 , HRP conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-T7 HRP polyclonal antibody 5237
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000- 1:30,000. Immunocytochemistry: use at a dilution of 1:200-1:500 ELISA: use at a dilution of 1:10,000- 1:100,000 These are recommended dilutions. Endusers should determine optimal dilutions for their applications. |
| Immunogen T7 (MASMTGGQQMG) conjugated to KLH. |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| T7 tag |
| Protein names T7 epitope tag |
| Alternative names T7 tag, T7 epitope, Gene 10 tag, MASMTGGQQMG tag |
| Function The T7 tag is an artificial peptide epitope used for detection and characterization of recombinant proteins using anti-T7 tag antibodies. |
| Description The T7 tag is a peptide epitope derived from the major capsid protein (gene 10 protein) of bacteriophage T7. It is commonly used as a recombinant protein tag for detection, purification, and characterization of fusion proteins. |
| Keywords Epitope tag, Protein detection, Protein purification, Immunoprecipitation, Recombinant protein |
| Sequence MASMTGGQQMG |
| Structure The T7 tag is an 11-amino acid peptide epitope with the sequence MASMTGGQQMG derived from the bacteriophage T7 gene 10 major capsid protein. |
| Function The T7 tag is used as a molecular epitope tag for detection, purification, and characterization of recombinant proteins. |
| Detection T7-tagged proteins can be detected using anti-T7 tag antibodies in western blotting, ELISA, immunofluorescence, immunocytochemistry, and immunoprecipitation assays. |
| Applications The T7 tag is commonly incorporated into expression vectors and fused to recombinant proteins to facilitate detection and purification. |
| Research relevance The T7 tag is widely used in bacterial expression systems and molecular biology workflows and serves as an alternative to FLAG, HA, Myc, V5, and His tags. |
Data
 |
| Detection of T7-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-T7 antibody 5237 used for WB at 0.2 µg /ml (1:5,000). Detection: Chemiluminescence with an exposure time of 30 seconds. |
FAQ & Publications
Frequently Asked Questions
What are the recommended dilution ranges for the goat anti-T7 HRP polyclonal antibody in various applications?
For immunoblotting (Western blot), use at a dilution of 1:1,000 to 1:30,000. For immunocytochemistry, a dilution of 1:200 to 1:500 is recommended. For ELISA, use at a dilution of 1:10,000 to 1:100,000. Optimal dilutions should be determined by the end user based on their specific experimental conditions.
What is the immunogen used to generate the goat anti-T7 HRP polyclonal antibody (SKU 5237)?
The immunogen is the T7 peptide sequence MASMTGGQQMG conjugated to Keyhole Limpet Hemocyanin (KLH).
How should the goat anti-T7 HRP polyclonal antibody be stored to maintain stability?
This antibody should be stored at 2-8°C, where it remains stable for at least one year. It is supplied in Phosphate Buffered Saline (PBS) containing 0.2% stabilizer.
Which host species and clonality characterize this anti-T7 antibody?
The antibody is a goat-derived polyclonal antibody of the IgG isotype.
Is the goat anti-T7 HRP polyclonal antibody compatible with other secondary antibodies for detection?
Yes, it is compatible with donkey anti-goat IgG secondary antibodies conjugated to peroxidase, biotin, or FITC, including H&L chain specific and cross-absorbed polyclonal antibodies.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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