| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Mouse IgM |
| available sizes | 1 mg |
goat anti-IgM-Alkaline Phosphatase Conjugate polyclonal antibody 9235
$211.00
Antibody summary
- Goat polyclonal to Mouse IgM-Alkaline Phosphatase Conjugate
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-IgM-Alkaline Phosphatase Conjugate polyclonal antibody 9235
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:10,000. Immunohistochemistry: use at a dilution of 1:100-1:1,000 Immunocytochemistry: use at a dilution of 1:100-1:1,000 ELISA: use at a dilution of 1:1,000-1:10,000. These are recommended dilutions. End users sh |
| Immunogen Mouse IgM |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least 1 year at 2-8°C. |
| Storage buffer 50 mM HEPES pH 7.1, 0.1 M NaCl, 1 mM MgCl2, 0.1 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| mouse IgM |
| Protein names Immunoglobulin M |
| Alternative names IgM, M immunoglobulin, Mu immunoglobulin |
| Function IgM provides early immune protection by recognizing pathogens, agglutinating antigens, activating the classical complement pathway, and facilitating pathogen clearance. |
| Subcellular location Secreted; present in blood plasma, lymph, and extracellular fluids. Membrane-bound forms are expressed on B lymphocytes. |
| Structure Secreted IgM typically exists as a pentamer consisting of five immunoglobulin subunits joined by disulfide bonds and a joining (J) chain. Membrane-bound IgM functions as the B-cell antigen receptor on naïve B lymphocytes. |
| Description Immunoglobulin M (IgM) is the first antibody isotype produced during a primary immune response. It is highly effective at antigen binding and complement activation and serves as a critical component of early humoral immunity. |
| Related entities relationship: binds_to; entity: C1q relationship: related_to; entity: Immunoglobulin G relationship: related_to; entity: Immunoglobulin A relationship: related_to; entity: Immunoglobulin E relationship: has_component; entity: J chain |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Primary immune response, Complement activation |
| Structure Secreted IgM is typically a pentamer composed of five antibody subunits linked by disulfide bonds and a joining (J) chain. Membrane-bound IgM is expressed as a monomer on B cells. |
| Function IgM provides rapid protection during the early stages of infection through high-avidity antigen binding, agglutination, and complement activation. |
| Complement activation IgM is the most potent activator of the classical complement pathway and efficiently recruits C1q following antigen binding. |
| Clinical relevance Detection of pathogen-specific IgM is commonly used as evidence of recent or acute infection. IgM measurements are widely used in infectious disease, autoimmune disease, and vaccine response testing. |
| Research relevance IgM is extensively studied in immunology, infectious disease, vaccine development, B-cell biology, and complement-mediated immunity. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What species does the goat anti-IgM-Alkaline Phosphatase Conjugate polyclonal antibody 9235 specifically recognize?
This antibody specifically reacts with Mouse IgM, making it suitable for applications involving mouse samples.
What are the recommended dilution ranges for using this antibody in immunohistochemistry and ELISA assays?
For immunohistochemistry (IHC), the recommended dilution range is 1:100 to 1:1,000. For ELISA applications, use the antibody at a dilution between 1:1,000 and 1:10,000.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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