| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Human IgM |
| available sizes | 1 mg |
goat anti-Human IgM, Peroxidase Conjugated polyclonal antibody 4485
$267.00
Antibody summary
- Goat polyclonal to Human IgM, Peroxidase Conjugated
- Suitable for: WB,IHC,ICC,ELISA
- Isotype: Whole IgG
- 1 mg
goat anti-Human IgM, Peroxidase Conjugated polyclonal antibody 4485
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,ELISA |
| Recommended dilutions ELISA: 1:10,000 1 1:100,000 Western Blot: 1:1,000 - 1:30,000 Immunohistochemistry: 1:200 - 1:500 Immunocytochemistry: 1:200 - 1:500 These are recommended dilutions. End user should determine optimal dilution for their application. |
| Immunogen Purified human IgM |
| Size and concentration 1mg and 1 mg/mL |
| Form liquid |
| Storage Instructions Store at 2-8°C. Stable for 1 year from date of receipt. |
| Storage buffer Phosphate Buffered Saline (PBS) containing 0.2% |
| Purity peptide affinity purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| human IgM |
| Protein names Immunoglobulin M |
| Alternative names IgM, M immunoglobulin, Mu immunoglobulin |
| Function IgM provides early immune protection by recognizing pathogens, agglutinating antigens, activating the classical complement pathway, and facilitating pathogen clearance. |
| Subcellular location Secreted; present in blood plasma, lymph, and extracellular fluids. Membrane-bound forms are expressed on B lymphocytes. |
| Structure Secreted IgM typically exists as a pentamer consisting of five immunoglobulin subunits joined by disulfide bonds and a joining (J) chain. Membrane-bound IgM functions as the B-cell antigen receptor on naïve B lymphocytes. |
| Description Immunoglobulin M (IgM) is the first antibody isotype produced during a primary immune response. It is highly effective at antigen binding and complement activation and serves as a critical component of early humoral immunity. |
| Related entities relationship: binds_to; entity: C1q relationship: related_to; entity: Immunoglobulin G relationship: related_to; entity: Immunoglobulin A relationship: related_to; entity: Immunoglobulin E relationship: has_component; entity: J chain |
| Keywords Antibody, Immunoglobulin, Humoral immunity, Primary immune response, Complement activation |
| Structure Secreted IgM is typically a pentamer composed of five antibody subunits linked by disulfide bonds and a joining (J) chain. Membrane-bound IgM is expressed as a monomer on B cells. |
| Function IgM provides rapid protection during the early stages of infection through high-avidity antigen binding, agglutination, and complement activation. |
| Complement activation IgM is the most potent activator of the classical complement pathway and efficiently recruits C1q following antigen binding. |
| Clinical relevance Detection of pathogen-specific IgM is commonly used as evidence of recent or acute infection. IgM measurements are widely used in infectious disease, autoimmune disease, and vaccine response testing. |
| Research relevance IgM is extensively studied in immunology, infectious disease, vaccine development, B-cell biology, and complement-mediated immunity. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What species does the goat anti-Human IgM peroxidase conjugated antibody 4485 specifically recognize?
This antibody specifically reacts with human Immunoglobulin M (IgM).
Which applications is this goat anti-Human IgM antibody suitable for?
The antibody is validated for use in Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry/Immunofluorescence (ICC/IF), and ELISA assays.
How should the goat anti-Human IgM, Peroxidase Conjugated antibody be stored to ensure stability?
Store the antibody at 2-8°C, where it remains stable for one year from the date of receipt.
What is the recommended dilution range for using this antibody in ELISA and Western Blot applications?
For ELISA, the recommended dilution is between 1:10,000 and 1:100,000. For Western Blot, the suggested dilution range is 1:1,000 to 1:30,000, though optimal dilutions should be empirically determined by the end user.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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