| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | goat |
| isotype | IgG |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Glutathione-S-Transferase (GST) |
| available sizes | 100 µg |
goat anti-Glutathione-S-Transferase (GST) polyclonal antibody 6860
$271.00
Antibody summary
- Goat polyclonal to Glutathione-S-Transferase (GST)
- Suitable for: WB,IP,ICC,ELISA
- Isotype: Whole IgG
- 100 µg
goat anti-Glutathione-S-Transferase (GST) polyclonal antibody 6860
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA,IP |
| Recommended dilutions Immunoblotting: use at a dilution of 1:1,000-1:20,000. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use 1-4ug/mg lysate ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. These ar |
| Immunogen Glutathione-S-Transferase (GST) from Schistosoma japonicum. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at 2-8°C. |
| Storage buffer PBS, pH 7.2, 0.09% NaN3. |
| Purity GST purification |
| Clonality polyclonal |
| Isotype IgG |
| Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
| Isotype control Goat polyclonal - Isotype Control |
| target relevance |
|---|
| GST |
| Protein names Glutathione S-transferase tag |
| Alternative names GST tag, GST fusion tag, Schistosoma japonicum GST |
| Function The GST tag is fused to recombinant proteins to facilitate purification, detection, immobilization, and analysis of protein-protein interactions through high-affinity binding to glutathione. |
| Structure GST is a soluble enzyme of approximately 26 kDa that forms a stable folded protein domain when fused to target proteins. |
| Description The GST tag is a recombinant protein fusion tag derived from Schistosoma japonicum glutathione S-transferase. It is widely used for affinity purification, protein detection, pull-down assays, and studies of protein-protein interactions. |
| Related entities relationship: binds_to; entity: Glutathione |
| Keywords Fusion tag, Affinity purification, Pull-down assay, Protein-protein interaction, Recombinant protein |
| Structure The GST tag is a 26 kDa protein derived from Schistosoma japonicum glutathione S-transferase. Unlike small epitope tags such as FLAG or HA, GST is a folded protein domain. |
| Function GST functions as an affinity tag that enables purification, detection, and immobilization of recombinant fusion proteins through binding to glutathione. |
| Purification GST-tagged proteins are typically purified using glutathione agarose or glutathione magnetic beads under native conditions. |
| Applications GST fusion proteins are widely used in western blotting, ELISA, affinity purification, pull-down assays, protein interaction studies, and recombinant protein production. |
| Research relevance GST is one of the most widely used recombinant fusion tags and is particularly valuable for protein purification and protein-protein interaction experiments. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications is the goat anti-Glutathione-S-Transferase (GST) polyclonal antibody 6860 validated for?
This antibody is suitable for Western blot (WB), immunoprecipitation (IP), immunocytochemistry/immunofluorescence (ICC/IF), and ELISA applications.
How should the goat anti-GST polyclonal antibody be stored to maintain stability?
The antibody should be stored at 2-8°C, where it remains stable for at least one year.
What is the recommended dilution range for using this antibody in immunoblotting?
For immunoblotting, use the antibody at dilutions ranging from 1:1,000 to 1:20,000.
From which species is the host of this anti-GST antibody derived?
The antibody is a goat polyclonal antibody, meaning it is produced in goats.
What is the immunogen used to generate the goat anti-Glutathione-S-Transferase polyclonal antibody 6860?
The immunogen is Glutathione-S-Transferase (GST) derived from Schistosoma japonicum.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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